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1bcm

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Revision as of 20:08, 24 December 2014

BACTERIOPHAGE MU TRANSPOSASE CORE DOMAIN WITH 2 MONOMERS PER ASYMMETRIC UNIT

Structural highlights

1bcm is a 2 chain structure with sequence from Enterobacteria phage mu. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	MUA (AMINO ACIDS 248 - 574) (Enterobacteria phage Mu)
Resources:	FirstGlance, OCA, RCSB, PDBsum

Function

[TRA_BPMU] This transposase is essential for integration, replication-transposition, and excision of Mu DNA.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The crystal structure of the core domain of bacteriophage Mu transposase, MuA, has been determined at 2.4 A resolution. The first of two subdomains contains the active site and, despite very limited sequence homology, exhibits a striking similarity to the core domain of HIV-1 integrase, which carries out a similar set of biochemical reactions. It also exhibits more limited similarity to other nucleases, RNase H and RuvC. The second, a beta barrel, connects to the first subdomain through several contacts. Three independent determinations of the monomer structure from two crystal forms all show the active site held in a similar, apparently inactive configuration. The enzymatic activity of MuA is known to be activated by formation of a DNA-bound tetramer of the protein. We propose that the connections between the two subdomains may be involved in the cross-talk between the active site and the other domains of the transposase that controls the activity of the protein.

Structure of the bacteriophage Mu transposase core: a common structural motif for DNA transposition and retroviral integration.,Rice P, Mizuuchi K Cell. 1995 Jul 28;82(2):209-20. PMID:7628012^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Rice P, Mizuuchi K. Structure of the bacteriophage Mu transposase core: a common structural motif for DNA transposition and retroviral integration. Cell. 1995 Jul 28;82(2):209-20. PMID:7628012

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1bcm"

@@ Line 6: / Line 6: @@
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1bcm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bcm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1bcm RCSB], [http://www.ebi.ac.uk/pdbsum/1bcm PDBsum]</span></td></tr>
 </table>
+== Function ==
+[[http://www.uniprot.org/uniprot/TRA_BPMU TRA_BPMU]] This transposase is essential for integration, replication-transposition, and excision of Mu DNA.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]

1bcm

From Proteopedia

Revision as of 20:08, 24 December 2014

BACTERIOPHAGE MU TRANSPOSASE CORE DOMAIN WITH 2 MONOMERS PER ASYMMETRIC UNIT

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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