1ty4
From Proteopedia
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|PDB= 1ty4 |SIZE=350|CAPTION= <scene name='initialview01'>1ty4</scene>, resolution 2.2Å | |PDB= 1ty4 |SIZE=350|CAPTION= <scene name='initialview01'>1ty4</scene>, resolution 2.2Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= ced-9,T07C4.8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 Caenorhabditis elegans]) | |GENE= ced-9,T07C4.8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 Caenorhabditis elegans]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ty4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ty4 OCA], [http://www.ebi.ac.uk/pdbsum/1ty4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ty4 RCSB]</span> | ||
}} | }} | ||
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[[Category: recognition]] | [[Category: recognition]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:02:40 2008'' |
Revision as of 21:02, 30 March 2008
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| , resolution 2.2Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | ced-9,T07C4.8 (Caenorhabditis elegans) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of a CED-9/EGL-1 complex
Overview
Programmed cell death in Caenorhabditis elegans is initiated by the binding of EGL-1 to CED-9, which disrupts the CED-4/CED-9 complex and allows CED-4 to activate the cell-killing caspase CED-3. Here we demonstrate that the C-terminal half of EGL-1 is necessary and sufficient for binding to CED-9 and for killing cells. Structure of the EGL-1/CED-9 complex revealed that EGL-1 adopts an extended alpha-helical conformation and induces substantial structural rearrangements in CED-9 upon binding. EGL-1 interface mutants failed to bind to CED-9 or to release CED-4 from the CED-4/CED-9 complex, and were unable to induce cell death in vivo. A surface patch on CED-9, different from that required for binding to EGL-1, was identified to be responsible for binding to CED-4. These data suggest a working mechanism for the release of CED-4 from the CED-4/CED-9 complex upon EGL-1 binding and provide a mechanistic framework for understanding apoptosis activation in C. elegans.
About this Structure
1TY4 is a Protein complex structure of sequences from Caenorhabditis elegans. Full crystallographic information is available from OCA.
Reference
Structural, biochemical, and functional analyses of CED-9 recognition by the proapoptotic proteins EGL-1 and CED-4., Yan N, Gu L, Kokel D, Chai J, Li W, Han A, Chen L, Xue D, Shi Y, Mol Cell. 2004 Sep 24;15(6):999-1006. PMID:15383288
Page seeded by OCA on Mon Mar 31 00:02:40 2008
Categories: Caenorhabditis elegans | Protein complex | Gu, L. | Kokel, D. | Shi, Y. | Xue, D. | Yan, N. | Apoptosis | Bcl-2 family protein | Ced-9 | Egl-1 | Recognition
