1tzy
From Proteopedia
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|PDB= 1tzy |SIZE=350|CAPTION= <scene name='initialview01'>1tzy</scene>, resolution 1.90Å | |PDB= 1tzy |SIZE=350|CAPTION= <scene name='initialview01'>1tzy</scene>, resolution 1.90Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1hq3|1hq3]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tzy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tzy OCA], [http://www.ebi.ac.uk/pdbsum/1tzy PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1tzy RCSB]</span> | ||
}} | }} | ||
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[[Category: Reynolds, C D.]] | [[Category: Reynolds, C D.]] | ||
[[Category: Wood, C M.]] | [[Category: Wood, C M.]] | ||
| - | [[Category: CL]] | ||
| - | [[Category: PO4]] | ||
[[Category: histone-fold]] | [[Category: histone-fold]] | ||
[[Category: tetramer-dimer-dimer]] | [[Category: tetramer-dimer-dimer]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:03:29 2008'' |
Revision as of 21:03, 30 March 2008
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| , resolution 1.90Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Related: | 1hq3
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the Core-Histone Octamer to 1.90 Angstrom Resolution
Overview
Crystals of native histone octamers (H2A-H2B)-(H4-H3)-(H3'-H4')-(H2B'-H2A') from chick erythrocytes in 2 M KCl, 1.35 M potassium phosphate pH 6.9 diffract X-rays to 1.90 A resolution, yielding a structure with an R(work) value of 18.7% and an Rfree of 22.2%. The crystal space group is P6(5), the asymmetric unit of which contains one complete octamer. This high-resolution model of the histone-core octamer allows further insight into intermolecular interactions, including water molecules, that dock the histone dimers to the tetramer in the nucleosome-core particle and have relevance to nucleosome remodelling. The three key areas analysed are the H2A'-H3-H4 molecular cluster (also H2A-H3'-H4'), the H4-H2B' interaction (also H4'-H2B) and the H2A'-H4 beta-sheet interaction (also H2A-H4'). The latter of these three regions is important to nucleosome remodelling by RNA polymerase II, as it is shown to be a likely core-histone binding site, and its disruption creates an instability in the nucleosome-core particle. A majority of the water molecules in the high-resolution octamer have positions that correlate to similar positions in the high-resolution nucleosome-core particle structure, suggesting that the high-resolution octamer model can be used for comparative studies with the high-resolution nucleosome-core particle.
About this Structure
1TZY is a Protein complex structure of sequences from Gallus gallus. Full crystallographic information is available from OCA.
Reference
High-resolution structure of the native histone octamer., Wood CM, Nicholson JM, Lambert SJ, Chantalat L, Reynolds CD, Baldwin JP, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jun 1;61(Pt, 6):541-5. Epub 2005 Jun 1. PMID:16511091
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