1u0o
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= VwF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]) | |GENE= VwF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1auq|1AUQ]], [[1ijb|1IJB]], [[1ijk|1IJK]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0o OCA], [http://www.ebi.ac.uk/pdbsum/1u0o PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1u0o RCSB]</span> | ||
}} | }} | ||
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[[Category: rossmann fold]] | [[Category: rossmann fold]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:03:45 2008'' |
Revision as of 21:03, 30 March 2008
| |||||||
| , resolution 2.70Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | VwF (Mus musculus) | ||||||
| Related: | 1AUQ, 1IJB, 1IJK
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The mouse von Willebrand Factor A1-botrocetin complex
Overview
Botrocetin is a snake venom protein that enhances the affinity of the A1 domain of plasma von Willebrand factor (vWF) for the platelet receptor glycoprotein Ibalpha (GPIbalpha), an event that contributes to bleeding and host death. Here we describe a kinetic and crystallographic analysis of this interaction that reveals a novel mechanism of affinity enhancement. Using high-temporal-resolution microscopy, we show that botrocetin decreases the GPIbalpha off-rate two-fold in both human and mouse complexes without affecting the on-rate. The key to this behavior is that, upon binding of GPIbalpha to vWF-A1, botrocetin prebound to vWF-A1 makes no contacts initially with GPIbalpha, but subsequently slides around the A1 surface to form a new interface. This two-step mechanism and flexible coupling may prevent adverse alterations in on-rate of GPIbalpha for vWF-A1, and permit adaptation to structural differences in GPIbalpha and vWF in several prey species.
About this Structure
1U0O is a Protein complex structure of sequences from Bothrops jararaca and Mus musculus. Full crystallographic information is available from OCA.
Reference
The snake venom protein botrocetin acts as a biological brace to promote dysfunctional platelet aggregation., Fukuda K, Doggett T, Laurenzi IJ, Liddington RC, Diacovo TG, Nat Struct Mol Biol. 2005 Feb;12(2):152-9. Epub 2005 Jan 16. PMID:15665869
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