1ua1
From Proteopedia
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|PDB= 1ua1 |SIZE=350|CAPTION= <scene name='initialview01'>1ua1</scene>, resolution 2.00Å | |PDB= 1ua1 |SIZE=350|CAPTION= <scene name='initialview01'>1ua1</scene>, resolution 2.00Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=AF:2-AMINOFLUORENE'>AF</scene>, <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1ua0|1UA0]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ua1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ua1 OCA], [http://www.ebi.ac.uk/pdbsum/1ua1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ua1 RCSB]</span> | ||
}} | }} | ||
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[[Category: Hsu, G W.]] | [[Category: Hsu, G W.]] | ||
[[Category: Kiefer, J R.]] | [[Category: Kiefer, J R.]] | ||
| - | [[Category: | + | [[Category: aminofluorene]] |
| - | [[Category: | + | [[Category: aromatic amine]] |
| - | [[Category: dna polymerase i | + | [[Category: dna lesion]] |
| + | [[Category: dna polymerase i]] | ||
| + | [[Category: dna replication]] | ||
| + | [[Category: klenow fragment]] | ||
| + | [[Category: protein-dna complex]] | ||
| + | [[Category: translation replication]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:07:24 2008'' |
Revision as of 21:07, 30 March 2008
| |||||||
| , resolution 2.00Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , , | ||||||
| Activity: | DNA-directed DNA polymerase, with EC number 2.7.7.7 | ||||||
| Related: | 1UA0
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of aminofluorene adduct paired opposite cytosine at the polymerase active site.
Overview
Aromatic amines have been studied for more than a half-century as model carcinogens representing a class of chemicals that form bulky adducts to the C8 position of guanine in DNA. Among these guanine adducts, the N-(2'-deoxyguanosin-8-yl)-aminofluorene (G-AF) and N-2-(2'-deoxyguanosin-8-yl)-acetylaminofluorene (G-AAF) derivatives are the best studied. Although G-AF and G-AAF differ by only an acetyl group, they exert different effects on DNA replication by replicative and high-fidelity DNA polymerases. Translesion synthesis of G-AF is achieved with high-fidelity polymerases, whereas replication of G-AAF requires specialized bypass polymerases. Here we have presented structures of G-AF as it undergoes one round of accurate replication by a high-fidelity DNA polymerase. Nucleotide incorporation opposite G-AF is achieved in solution and in the crystal, revealing how the polymerase accommodates and replicates past G-AF, but not G-AAF. Like an unmodified guanine, G-AF adopts a conformation that allows it to form Watson-Crick hydrogen bonds with an opposing cytosine that results in protrusion of the bulky fluorene moiety into the major groove. Although incorporation opposite G-AF is observed, the C:G-AF base pair induces distortions to the polymerase active site that slow translesion synthesis.
About this Structure
1UA1 is a Protein complex structure of sequences from Geobacillus stearothermophilus. Full crystallographic information is available from OCA.
Reference
Observing translesion synthesis of an aromatic amine DNA adduct by a high-fidelity DNA polymerase., Hsu GW, Kiefer JR, Burnouf D, Becherel OJ, Fuchs RP, Beese LS, J Biol Chem. 2004 Nov 26;279(48):50280-5. Epub 2004 Sep 22. PMID:15385534
Page seeded by OCA on Mon Mar 31 00:07:24 2008
Categories: DNA-directed DNA polymerase | Geobacillus stearothermophilus | Protein complex | Becherel, O J. | Beese, L S. | Fuchs, R P.P. | Hsu, G W. | Kiefer, J R. | Aminofluorene | Aromatic amine | Dna lesion | Dna polymerase i | Dna replication | Klenow fragment | Protein-dna complex | Translation replication
