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1uk8

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|PDB= 1uk8 |SIZE=350|CAPTION= <scene name='initialview01'>1uk8</scene>, resolution 1.60&Aring;
|PDB= 1uk8 |SIZE=350|CAPTION= <scene name='initialview01'>1uk8</scene>, resolution 1.60&Aring;
|SITE=
|SITE=
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|LIGAND= <scene name='pdbligand=LEA:LEVULINIC ACID'>LEA</scene>
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|LIGAND= <scene name='pdbligand=LEA:LEVULINIC+ACID'>LEA</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/2-hydroxymuconate-semialdehyde_hydrolase 2-hydroxymuconate-semialdehyde hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.7.1.9 3.7.1.9]
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/2-hydroxymuconate-semialdehyde_hydrolase 2-hydroxymuconate-semialdehyde hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.7.1.9 3.7.1.9] </span>
|GENE= CUMD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=294 Pseudomonas fluorescens])
|GENE= CUMD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=294 Pseudomonas fluorescens])
 +
|DOMAIN=
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|RELATEDENTRY=[[1iun|1IUN]], [[1iuo|1IUO]], [[1iup|1IUP]], [[1uk6|1UK6]], [[1uk7|1UK7]], [[1uk9|1UK9]], [[1uka|1UKA]], [[1ukb|1UKB]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uk8 OCA], [http://www.ebi.ac.uk/pdbsum/1uk8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1uk8 RCSB]</span>
}}
}}
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[[Category: Wakagi, T.]]
[[Category: Wakagi, T.]]
[[Category: Yamane, H.]]
[[Category: Yamane, H.]]
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[[Category: LEA]]
 
[[Category: alpha/beta-hydrolase]]
[[Category: alpha/beta-hydrolase]]
[[Category: aromatic compound]]
[[Category: aromatic compound]]
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[[Category: substrate specificity]]
[[Category: substrate specificity]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:33:02 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:11:25 2008''

Revision as of 21:11, 30 March 2008

Image:1uk8.jpg


PDB ID 1uk8

Drag the structure with the mouse to rotate
, resolution 1.60Å
Ligands:
Gene: CUMD (Pseudomonas fluorescens)
Activity: 2-hydroxymuconate-semialdehyde hydrolase, with EC number 3.7.1.9
Related: 1IUN, 1IUO, 1IUP, 1UK6, 1UK7, 1UK9, 1UKA, 1UKB


Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Crystal structure of a meta-cleavage product hydrolase (CumD) complexed with n-valerate


Overview

Meta-cleavage product hydrolase (MCP-hydrolase) is one of the key enzymes in the microbial degradation of aromatic compounds. MCP-hydrolase produces 2-hydroxypenta-2,4-dienoate and various organic acids, according to the C6 substituent of the substrate. Comprehensive analysis of the substrate specificity of the MCP-hydrolase from Pseudomonas fluorescens IP01 (CumD) was carried out by determining the kinetic parameters for nine substrates and crystal structures complexed with eight cleavage products. CumD preferred substrates with long non-branched C6 substituents, but did not effectively hydrolyze a substrate with a phenyl group. Superimposition of the complex structures indicated that benzoate was bound in a significantly different direction than other aliphatic cleavage products. The directions of the bound organic acids appeared to be related with the k(cat) values of the corresponding substrates. The Ile139 and Trp143 residues on helix alpha4 appeared to cause steric hindrance with the aromatic ring of the substrate, which hampers base-catalyzed attack by water.

About this Structure

1UK8 is a Single protein structure of sequence from Pseudomonas fluorescens. Full crystallographic information is available from OCA.

Reference

A series of crystal structures of a meta-cleavage product hydrolase from Pseudomonas fluorescens IP01 (CumD) complexed with various cleavage products., Fushinobu S, Jun SY, Hidaka M, Nojiri H, Yamane H, Shoun H, Omori T, Wakagi T, Biosci Biotechnol Biochem. 2005 Mar;69(3):491-8. PMID:15784976

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