1uyo
From Proteopedia
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|PDB= 1uyo |SIZE=350|CAPTION= <scene name='initialview01'>1uyo</scene>, resolution 3.20Å | |PDB= 1uyo |SIZE=350|CAPTION= <scene name='initialview01'>1uyo</scene>, resolution 3.20Å | ||
|SITE= <scene name='pdbsite=AC1:Cxe+Binding+Site+For+Chain+X'>AC1</scene> | |SITE= <scene name='pdbsite=AC1:Cxe+Binding+Site+For+Chain+X'>AC1</scene> | ||
| - | |LIGAND= <scene name='pdbligand=CXE:PENTAETHYLENE GLYCOL MONODECYL ETHER'>CXE</scene> | + | |LIGAND= <scene name='pdbligand=CXE:PENTAETHYLENE+GLYCOL+MONODECYL+ETHER'>CXE</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uyo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uyo OCA], [http://www.ebi.ac.uk/pdbsum/1uyo PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1uyo RCSB]</span> | ||
}} | }} | ||
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[[Category: Tommassen, J.]] | [[Category: Tommassen, J.]] | ||
[[Category: Ulsen, P Van.]] | [[Category: Ulsen, P Van.]] | ||
| - | [[Category: CXE]] | ||
[[Category: autotransporter]] | [[Category: autotransporter]] | ||
[[Category: beta-barrel]] | [[Category: beta-barrel]] | ||
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[[Category: translocator domain]] | [[Category: translocator domain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:17:13 2008'' |
Revision as of 21:17, 30 March 2008
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| , resolution 3.20Å | |||||||
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| Ligands: | , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
TRANSLOCATOR DOMAIN OF AUTOTRANSPORTER NALP FROM NEISSERIA MENINGITIDIS
Overview
Autotransporters are virulence-related proteins of Gram-negative bacteria that are secreted via an outer-membrane-based C-terminal extension, the translocator domain. This domain supposedly is sufficient for the transport of the N-terminal passenger domain across the outer membrane. We present here the crystal structure of the in vitro-folded translocator domain of the autotransporter NalP from Neisseria meningitidis, which reveals a 12-stranded beta-barrel with a hydrophilic pore of 10 x 12.5 A that is filled by an N-terminal alpha-helix. The domain has pore activity in vivo and in vitro. Our data are consistent with the model of passenger-domain transport through the hydrophilic channel within the beta-barrel, and inconsistent with a model for transport through a central channel formed by an oligomer of translocator domains. However, the dimensions of the pore imply translocation of the secreted domain in an unfolded form. An alternative model, possibly covering the transport of folded domains, is that passenger-domain transport involves the Omp85 complex, the machinery required for membrane insertion of outer-membrane proteins, on which autotransporters are dependent.
About this Structure
1UYO is a Single protein structure of sequence from Neisseria meningitidis. Full crystallographic information is available from OCA.
Reference
Structure of the translocator domain of a bacterial autotransporter., Oomen CJ, van Ulsen P, van Gelder P, Feijen M, Tommassen J, Gros P, EMBO J. 2004 Mar 24;23(6):1257-66. Epub 2004 Mar 11. PMID:15014442
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