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1fzr

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Revision as of 04:33, 25 December 2014

CRYSTAL STRUCTURE OF BACTERIOPHAGE T7 ENDONUCLEASE I

Structural highlights

1fzr is a 4 chain structure with sequence from Enterobacteria phage t7. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Activity:	Deoxyribonuclease IV (phage-T(4)-induced), with EC number 3.1.21.2
Resources:	FirstGlance, OCA, RCSB, PDBsum

Function

[ENRN_BPT7] Junction-resolving enzyme that selectively binds and cleaves four-way (Holliday) DNA junctions present after viral genomic replication. These intermediates are created during DNA repair, processing of stalled replication forks and homologous genetic recombination. Introduces two nicks on the two non-crossing strands, at 5' sides of the junction. Participates also together with gp6 in the degradation of host chromosome to provide nucleotides for phage DNA synthesis.^[1] ^[2] ^[3] ^[4]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

We have solved the crystal structure of the Holliday junction resolving enzyme T7 endonuclease I at 2.1 A resolution using the multiwavelength anomalous dispersion (MAD) technique. Endonuclease I exhibits strong structural specificity for four-way DNA junctions. The structure shows that it forms a symmetric homodimer arranged in two well-separated domains. Each domain, however, is composed of elements from both subunits, and amino acid side chains from both protomers contribute to the active site. While no significant structural similarity could be detected with any other junction resolving enzyme, the active site is similar to that found in several restriction endonucleases. T7 endonuclease I therefore represents the first crystal structure of a junction resolving enzyme that is a member of the nuclease superfamily of enzymes.

Crystal structure of the Holliday junction resolving enzyme T7 endonuclease I.,Hadden JM, Convery MA, Declais AC, Lilley DM, Phillips SE Nat Struct Biol. 2001 Jan;8(1):62-7. PMID:11135673^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Panayotatos N, Fontaine A. An endonuclease specific for single-stranded DNA selectively damages the genomic DNA and induces the SOS response. J Biol Chem. 1985 Mar 10;260(5):3173-7. PMID:3972821
↑ Parkinson MJ, Lilley DM. The junction-resolving enzyme T7 endonuclease I: quaternary structure and interaction with DNA. J Mol Biol. 1997 Jul 11;270(2):169-78. PMID:9236119 doi:http://dx.doi.org/10.1006/jmbi.1997.1128
↑ Declais AC, Fogg JM, Freeman AD, Coste F, Hadden JM, Phillips SE, Lilley DM. The complex between a four-way DNA junction and T7 endonuclease I. EMBO J. 2003 Mar 17;22(6):1398-409. PMID:12628932 doi:http://dx.doi.org/10.1093/emboj/cdg132
↑ Freeman AD, Declais AC, Lilley DM. The importance of the N-terminus of T7 endonuclease I in the interaction with DNA junctions. J Mol Biol. 2013 Jan 23;425(2):395-410. doi: 10.1016/j.jmb.2012.11.029. Epub 2012, Dec 1. PMID:23207296 doi:http://dx.doi.org/10.1016/j.jmb.2012.11.029
↑ Hadden JM, Convery MA, Declais AC, Lilley DM, Phillips SE. Crystal structure of the Holliday junction resolving enzyme T7 endonuclease I. Nat Struct Biol. 2001 Jan;8(1):62-7. PMID:11135673 doi:10.1038/83067

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1fzr"

@@ Line 6: / Line 6: @@
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1fzr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1fzr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1fzr RCSB], [http://www.ebi.ac.uk/pdbsum/1fzr PDBsum]</span></td></tr>
 </table>
+== Function ==
+[[http://www.uniprot.org/uniprot/ENRN_BPT7 ENRN_BPT7]] Junction-resolving enzyme that selectively binds and cleaves four-way (Holliday) DNA junctions present after viral genomic replication. These intermediates are created during DNA repair, processing of stalled replication forks and homologous genetic recombination. Introduces two nicks on the two non-crossing strands, at 5' sides of the junction. Participates also together with gp6 in the degradation of host chromosome to provide nucleotides for phage DNA synthesis.<ref>PMID:3972821</ref> <ref>PMID:9236119</ref> <ref>PMID:12628932</ref> <ref>PMID:23207296</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]

1fzr

From Proteopedia

Revision as of 04:33, 25 December 2014

CRYSTAL STRUCTURE OF BACTERIOPHAGE T7 ENDONUCLEASE I

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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