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1v07
From Proteopedia
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|PDB= 1v07 |SIZE=350|CAPTION= <scene name='initialview01'>1v07</scene>, resolution 1.70Å | |PDB= 1v07 |SIZE=350|CAPTION= <scene name='initialview01'>1v07</scene>, resolution 1.70Å | ||
|SITE= <scene name='pdbsite=AC1:So4+Environment+In+Chain+A'>AC1</scene> | |SITE= <scene name='pdbsite=AC1:So4+Environment+In+Chain+A'>AC1</scene> | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=OXY:OXYGEN+MOLECULE'>OXY</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1v07 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v07 OCA], [http://www.ebi.ac.uk/pdbsum/1v07 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1v07 RCSB]</span> | ||
}} | }} | ||
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[[Category: Pesce, A.]] | [[Category: Pesce, A.]] | ||
[[Category: Riggs, A.]] | [[Category: Riggs, A.]] | ||
| - | [[Category: HEM]] | ||
| - | [[Category: OXY]] | ||
| - | [[Category: SO4]] | ||
[[Category: nerve tissue mini-hemoglobin]] | [[Category: nerve tissue mini-hemoglobin]] | ||
[[Category: oxygen affinity of c lacteus mini-hemoglobin]] | [[Category: oxygen affinity of c lacteus mini-hemoglobin]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:17:48 2008'' |
Revision as of 21:17, 30 March 2008
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| , resolution 1.70Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Ligands: | , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
CRYSTAL STRUCTURE OF THRE11VAL MUTANT OF THE NERVE TISSUE MINI-HEMOGLOBIN FROM THE NEMERTEAN WORM CEREBRATULUS LACTEUS
Overview
The mini-hemoglobin from Cerebratulus lacteus (CerHb) belongs to a class of globins containing the polar Tyr-B10/Gln-E7 amino acid pair that normally causes low rates of O2 dissociation and ultra-high O2 affinity, which suggest O2 sensing or NO scavenging functions. CerHb, however, has high rates of O2 dissociation (kO2 = 200-600 s(-1)) and moderate O2 affinity (KO2) approximately 1 microm(-1)) as a result of a third polar amino acid in its active site, Thr-E11. When Thr-E11 is replaced by Val, kO2 decreases 1000-fold and KO2 increases 130-fold at pH 7.0, 20 degrees C. The mutation also shifts the stretching frequencies of both heme-bound and photodissociated CO, indicating marked changes of the electrostatic field at the active site. The crystal structure of Thr-E11 --> Val CerHbO2 at 1.70 A resolution is almost identical to that of the wild-type protein (root mean square deviation of 0.12 A). The dramatic functional and spectral effects of the Thr-E11 --> Val mutation are due exclusively to changes in the hydrogen bonding network in the active site. Replacing Thr-E11 with Val "frees" the Tyr-B10 hydroxyl group to rotate toward and donate a strong hydrogen bond to the heme-bound ligand, causing a selective increase in O2 affinity, a decrease of the rate coefficient for O2 dissociation, a 40 cm(-1) decrease in nuCO of heme-bound CO, and an increase in ligand migration toward more remote intermediate sites.
About this Structure
1V07 is a Single protein structure of sequence from Cerebratulus lacteus. Full crystallographic information is available from OCA.
Reference
Thr-E11 regulates O2 affinity in Cerebratulus lacteus mini-hemoglobin., Pesce A, Nardini M, Ascenzi P, Geuens E, Dewilde S, Moens L, Bolognesi M, Riggs AF, Hale A, Deng P, Nienhaus GU, Olson JS, Nienhaus K, J Biol Chem. 2004 Aug 6;279(32):33662-72. Epub 2004 May 25. PMID:15161908
Page seeded by OCA on Mon Mar 31 00:17:48 2008
Categories: Cerebratulus lacteus | Single protein | Ascenzi, P. | Bolognesi, M. | Deng, P. | Dewilde, S. | Geuens, E. | Hale, A. | Moens, L. | Nardini, M. | Nienhaus, G U. | Nienhaus, K. | Olson, J S. | Pesce, A. | Riggs, A. | Nerve tissue mini-hemoglobin | Oxygen affinity of c lacteus mini-hemoglobin
