1vm1
From Proteopedia
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|PDB= 1vm1 |SIZE=350|CAPTION= <scene name='initialview01'>1vm1</scene>, resolution 2.02Å | |PDB= 1vm1 |SIZE=350|CAPTION= <scene name='initialview01'>1vm1</scene>, resolution 2.02Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=AKR:ACRYLIC+ACID'>AKR</scene>, <scene name='pdbligand=MA4:CYCLOHEXYL-HEXYL-BETA-D-MALTOSIDE'>MA4</scene>, <scene name='pdbligand=TAZ:TAZOBACTAM'>TAZ</scene>, <scene name='pdbligand=TBE:TAZOBACTAM+INTERMEDIATE'>TBE</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span> |
|GENE= BLA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 Klebsiella pneumoniae]) | |GENE= BLA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=573 Klebsiella pneumoniae]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1shv|1SHV]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vm1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vm1 OCA], [http://www.ebi.ac.uk/pdbsum/1vm1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1vm1 RCSB]</span> | ||
}} | }} | ||
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[[Category: Nukaga, M.]] | [[Category: Nukaga, M.]] | ||
[[Category: Nukaga, Y.]] | [[Category: Nukaga, Y.]] | ||
| - | [[Category: AKR]] | ||
| - | [[Category: MA4]] | ||
| - | [[Category: TAZ]] | ||
| - | [[Category: TBE]] | ||
[[Category: beta-lactam hydrolase]] | [[Category: beta-lactam hydrolase]] | ||
[[Category: beta-lactamase]] | [[Category: beta-lactamase]] | ||
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[[Category: penicillinase]] | [[Category: penicillinase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:26:15 2008'' |
Revision as of 21:26, 30 March 2008
| |||||||
| , resolution 2.02Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Gene: | BLA (Klebsiella pneumoniae) | ||||||
| Activity: | Beta-lactamase, with EC number 3.5.2.6 | ||||||
| Related: | 1SHV
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF SHV-1 BETA-LACTAMASE INHIBITED BY TAZOBACTAM
Overview
Two species resulting from the reaction of the SHV-1 class A beta-lactamase with the sulfone inhibitor tazobactam have been trapped at 100 K and mapped by X-ray crystallography at 2.0 A resolution. An acyclic form of tazobactam is covalently bonded to the catalytic Ser70 side chain, and a second species, a five-atom vinyl carboxylic acid fragment of tazobactam, is bonded to Ser130. It is proposed that the electron density map of the crystal is a composite picture of two complexes, each with only a single bound species. It is estimated that the two complexes exist in the crystal in approximately equal populations. Results are discussed in relation to the mechanism-based inhibition of class A beta-lactamases by the similar inhibitors sulbactam and clavulanic acid.
About this Structure
1VM1 is a Single protein structure of sequence from Klebsiella pneumoniae. This structure supersedes the now removed PDB entry 1G56. Full crystallographic information is available from OCA.
Reference
Inhibition of the SHV-1 beta-lactamase by sulfones: crystallographic observation of two reaction intermediates with tazobactam., Kuzin AP, Nukaga M, Nukaga Y, Hujer A, Bonomo RA, Knox JR, Biochemistry. 2001 Feb 13;40(6):1861-6. PMID:11327849
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