This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

4nj7

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 08:25, 4 August 2016

PB1 Domain of AtARF7 - SeMet Derivative

Structural highlights

4nj7 is a 16 chain structure with sequence from Arath. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:
Related:	4nj6
Gene:	ARF7, BIP, IAA21, IAA23, IAA25, NPH4, TIR5, At5g20730, T1M15.130 (ARATH)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ARFG_ARATH] Auxin response factors (ARFs) are transcriptional factors that binds specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Seems to act as transcriptional activator. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Required for differential growth responses of aerial tissues. Involved in ethylene responses. Regulates lateral root formation through direct regulation of LBD16 and/or LBD29. Functionally redundant with ARF19. Mediates embryo axis formation and vascular tissues differentiation. Functionally redundant with ARF7.^[1] ^[2] ^[3] ^[4]

Publication Abstract from PubMed

In plants, the AUXIN RESPONSE FACTOR (ARF) transcription factor family regulates gene expression in response to auxin. In the absence of auxin, ARF transcription factors are repressed by interaction with AUXIN/INDOLE 3-ACETIC ACID (Aux/IAA) proteins. Although the C termini of ARF and Aux/IAA proteins facilitate their homo- and heterooligomerization, the molecular basis for this interaction remained undefined. The crystal structure of the C-terminal interaction domain of Arabidopsis ARF7 reveals a Phox and Bem1p (PB1) domain that provides both positive and negative electrostatic interfaces for directional protein interaction. Mutation of interface residues in the ARF7 PB1 domain yields monomeric protein and abolishes interaction with both itself and IAA17. Expression of a stabilized Aux/IAA protein (i.e., IAA16) bearing PB1 mutations in Arabidopsis suggests a multimerization requirement for ARF protein repression, leading to a refined auxin-signaling model.

Molecular basis for AUXIN RESPONSE FACTOR protein interaction and the control of auxin response repression.,Korasick DA, Westfall CS, Lee SG, Nanao MH, Dumas R, Hagen G, Guilfoyle TJ, Jez JM, Strader LC Proc Natl Acad Sci U S A. 2014 Apr 8;111(14):5427-32. doi:, 10.1073/pnas.1400074111. Epub 2014 Mar 25. PMID:24706860^[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hagen G, Guilfoyle T. Auxin-responsive gene expression: genes, promoters and regulatory factors. Plant Mol Biol. 2002 Jun-Jul;49(3-4):373-85. PMID:12036261
↑ Hardtke CS, Ckurshumova W, Vidaurre DP, Singh SA, Stamatiou G, Tiwari SB, Hagen G, Guilfoyle TJ, Berleth T. Overlapping and non-redundant functions of the Arabidopsis auxin response factors MONOPTEROS and NONPHOTOTROPIC HYPOCOTYL 4. Development. 2004 Mar;131(5):1089-100. PMID:14973283 doi:http://dx.doi.org/10.1242/dev.00925
↑ Li J, Dai X, Zhao Y. A role for auxin response factor 19 in auxin and ethylene signaling in Arabidopsis. Plant Physiol. 2006 Mar;140(3):899-908. Epub 2006 Feb 3. PMID:16461383 doi:http://dx.doi.org/pp.105.070987
↑ Okushima Y, Fukaki H, Onoda M, Theologis A, Tasaka M. ARF7 and ARF19 regulate lateral root formation via direct activation of LBD/ASL genes in Arabidopsis. Plant Cell. 2007 Jan;19(1):118-30. Epub 2007 Jan 26. PMID:17259263 doi:http://dx.doi.org/tpc.106.047761
↑ Korasick DA, Westfall CS, Lee SG, Nanao MH, Dumas R, Hagen G, Guilfoyle TJ, Jez JM, Strader LC. Molecular basis for AUXIN RESPONSE FACTOR protein interaction and the control of auxin response repression. Proc Natl Acad Sci U S A. 2014 Apr 8;111(14):5427-32. doi:, 10.1073/pnas.1400074111. Epub 2014 Mar 25. PMID:24706860 doi:http://dx.doi.org/10.1073/pnas.1400074111

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4nj7"

@@ Line 1: / Line 1: @@
 ==PB1 Domain of AtARF7 - SeMet Derivative==
 <StructureSection load='4nj7' size='340' side='right' caption='[[4nj7]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4nj6|4nj6]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ARF7, BIP, IAA21, IAA23, IAA25, NPH4, TIR5, At5g20730, T1M15.130 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nj7 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4nj7 RCSB], [http://www.ebi.ac.uk/pdbsum/4nj7 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4nj7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nj7 OCA], [http://pdbe.org/4nj7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4nj7 RCSB], [http://www.ebi.ac.uk/pdbsum/4nj7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4nj7 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4nj7" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4nj7

From Proteopedia

Revision as of 08:25, 4 August 2016

PB1 Domain of AtARF7 - SeMet Derivative

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox