This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

3qsy

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 09:40, 4 August 2016

Recognition of the methionylated initiator tRNA by the translation initiation factor 2 in Archaea

Structural highlights

3qsy is a 3 chain structure with sequence from Atcc 35091 and Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Gene:	eif2g (ATCC 35091), eif2a (ATCC 35091)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[IF2G_SULSO] eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA (By similarity). [IF2A_SULSO] eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA (By similarity).

Publication Abstract from PubMed

Heterotrimeric aIF2alphabetagamma (archaeal homologue of the eukaryotic translation initiation factor 2) in its GTP-bound form delivers Met-tRNA(i)(Met) to the small ribosomal subunit. It is known that the heterodimer containing the GTP-bound gamma subunit and domain 3 of the alpha subunit of aIF2 is required for the formation of a stable complex with Met-tRNA(i). Here, the crystal structure of an incomplete ternary complex including aIF2alphaD3gammaGDPNPMet-tRNA(f)(Met) has been solved at 3.2A resolution. This structure is in good agreement with biochemical and hydroxyl radical probing data. The analysis of the complex shows that despite the structural similarity of aIF2gamma and the bacterial translation elongation factor EF-Tu, their modes of tRNA binding are very different. Remarkably, the recently published 5.0-A-resolution structure of almost the same ternary initiation complex differs dramatically from the structure presented. Reasons for this discrepancy are discussed.

Crystal Structure of the Archaeal Translation Initiation Factor 2 in Complex with a GTP Analogue and Met-tRNA(f)(Met).,Stolboushkina E, Nikonov S, Zelinskaya N, Arkhipova V, Nikulin A, Garber M, Nikonov O J Mol Biol. 2013 Jan 3. pii: S0022-2836(12)00971-0. doi:, 10.1016/j.jmb.2012.12.023. PMID:23291527^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Stolboushkina E, Nikonov S, Zelinskaya N, Arkhipova V, Nikulin A, Garber M, Nikonov O. Crystal Structure of the Archaeal Translation Initiation Factor 2 in Complex with a GTP Analogue and Met-tRNA(f)(Met). J Mol Biol. 2013 Jan 3. pii: S0022-2836(12)00971-0. doi:, 10.1016/j.jmb.2012.12.023. PMID:23291527 doi:10.1016/j.jmb.2012.12.023

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3qsy"

@@ Line 1: / Line 1: @@
 ==Recognition of the methionylated initiator tRNA by the translation initiation factor 2 in Archaea==
 <StructureSection load='3qsy' size='340' side='right' caption='[[3qsy]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MET:METHIONINE'>MET</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">eif2g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 ATCC 35091]), eif2a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 ATCC 35091])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qsy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qsy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qsy RCSB], [http://www.ebi.ac.uk/pdbsum/3qsy PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qsy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qsy OCA], [http://pdbe.org/3qsy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3qsy RCSB], [http://www.ebi.ac.uk/pdbsum/3qsy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3qsy ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3qsy" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[TRNA|TRNA]]
 == References ==
 <references/>

3qsy

From Proteopedia

Revision as of 09:40, 4 August 2016

Recognition of the methionylated initiator tRNA by the translation initiation factor 2 in Archaea

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox