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4tlp

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Revision as of 15:51, 5 August 2016

Crystal structure of a alanine91 mutant of WCI

Structural highlights

4tlp is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ICW3_PSOTE] Inhibits alpha-chymotrypsin at the molar ratio of 1:2 in state of 1:1.

Publication Abstract from PubMed

beta-trefoil fold, consisting of a six stranded beta-barrel capped at one end by a lid comprising of another six beta-strands, is one of the most important folds among proteins. Important classes of proteins like Interleukins (ILs), Fibroblast Growth Factors (FGFs), Kunitz (STI) family of inhibitors etc. belong to this fold. Their core is packed by hydrophobic residues contributed by the 6 stranded beta-barrel and three beta-hairpins that make essential contacts with each other and keep the protein in 'topologically minimal frustrated state'. A complete database analysis of the core residues of the beta-trefoil fold proteins presented here identified a conserved tryptophan (W91) residue in the Kunitz (STI) family of inhibitors that projects from the lid and interacts with the bottom layer residues of the barrel. This kind of interactions is unique in Kunitz (STI) family because no other families of beta-trefoil fold have such a shear sized residue at the barrel lid junction; suggesting its possible importance in packing and stability. We took WCI as a representative of this family and prepared four cavity creating mutants W91F-WCI, W91M-WCI, W91I-WCI & W91A-WCI. CD experiments show that the secondary structure of the mutants remains indistinguishable with the wild type. Crystal structures of the mutants W91F-WCI, W91M-WCI & W91A-WCI also show the same feature. However, slight readjustments of the side chains around the site of mutation have been observed so as to minimize the cavity created due to mutation. Comparative stability of these mutants, estimated using heat denaturation CD spectroscopy, indicates that stability of the mutants inversely correlates with the size of the cavity inside the core. Interestingly, although we mutated at the core, mutants show varying susceptibility against tryptic digestion that grossly follow their instability determined by CD. Our findings suggest that the W91 residue plays an important role in determining the stability and packing of the core of WCI.

A conserved tryptophan (W91) at the barrel-lid junction modulates the packing and stability of Kunitz (STI) family of inhibitors.,Majumder S, Khamrui S, Banerjee R, Bhowmik P, Sen U Biochim Biophys Acta. 2015 Jan;1854(1):55-64. doi: 10.1016/j.bbapap.2014.10.021. , Epub 2014 Oct 31. PMID:25448016^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Majumder S, Khamrui S, Banerjee R, Bhowmik P, Sen U. A conserved tryptophan (W91) at the barrel-lid junction modulates the packing and stability of Kunitz (STI) family of inhibitors. Biochim Biophys Acta. 2015 Jan;1854(1):55-64. doi: 10.1016/j.bbapap.2014.10.021. , Epub 2014 Oct 31. PMID:25448016 doi:http://dx.doi.org/10.1016/j.bbapap.2014.10.021

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4tlp"

@@ Line 1: / Line 1: @@
 ==Crystal structure of a alanine91 mutant of WCI==
 <StructureSection load='4tlp' size='340' side='right' caption='[[4tlp]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[4tlp]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TLP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4TLP FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tlp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4tlp RCSB], [http://www.ebi.ac.uk/pdbsum/4tlp PDBsum]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4tlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4tlp OCA], [http://pdbe.org/4tlp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4tlp RCSB], [http://www.ebi.ac.uk/pdbsum/4tlp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4tlp ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 15: / Line 16: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4tlp" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Chymotrypsin Inhibitor|Chymotrypsin Inhibitor]]
 == References ==
 <references/>

4tlp

From Proteopedia

Revision as of 15:51, 5 August 2016

Crystal structure of a alanine91 mutant of WCI

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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