4h8i

From Proteopedia

(Difference between revisions)

Revision as of 19:00, 4 August 2016

Structure of GluK2-LBD in complex with GluAzo

Structural highlights

4h8i is a 2 chain structure with sequence from Buffalo rat. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , ,
Related:	4h8j
Gene:	Glur6, Grik2 (Buffalo rat)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[GRIK2_RAT] Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. May be involved in the transmission of light information from the retina to the hypothalamus. Modulates cell surface expression of NETO2 (By similarity).^[1] ^[2]

Publication Abstract from PubMed

Incorporation of the azobenzene derivative gluazo, a synthetic photochromic ligand, into a kainate receptor allows for the optical control of neuronal activity. The crystal structure of gluazo bound to a dimeric GluK2 ligand-binding domain reveals one monomer in a closed conformation, occupied by gluazo, and the other in an open conformation, with a bound buffer molecule. The glutamate group of gluazo interacts like the natural glutamate ligand, while its trans-azobenzene moiety protrudes into a tunnel. This elongated cavity presumably cannot accommodate a cis-azobenzene, which explains the reversible activation of the receptor upon photoisomerization.

A photoswitchable neurotransmitter analogue bound to its receptor.,Reiter A, Skerra A, Trauner D, Schiefner A Biochemistry. 2013 Dec 17;52(50):8972-4. doi: 10.1021/bi4014402. Epub 2013 Dec 6. PMID:24295282^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Martin S, Nishimune A, Mellor JR, Henley JM. SUMOylation regulates kainate-receptor-mediated synaptic transmission. Nature. 2007 May 17;447(7142):321-5. Epub 2007 May 7. PMID:17486098 doi:nature05736
↑ Weston MC, Schuck P, Ghosal A, Rosenmund C, Mayer ML. Conformational restriction blocks glutamate receptor desensitization. Nat Struct Mol Biol. 2006 Dec;13(12):1120-7. Epub 2006 Nov 19. PMID:17115050 doi:http://dx.doi.org/10.1038/nsmb1178
↑ Reiter A, Skerra A, Trauner D, Schiefner A. A photoswitchable neurotransmitter analogue bound to its receptor. Biochemistry. 2013 Dec 17;52(50):8972-4. doi: 10.1021/bi4014402. Epub 2013 Dec 6. PMID:24295282 doi:http://dx.doi.org/10.1021/bi4014402

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4h8i"

@@ Line 1: / Line 1: @@
 ==Structure of GluK2-LBD in complex with GluAzo==
 <StructureSection load='4h8i' size='340' side='right' caption='[[4h8i]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4h8j|4h8j]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Glur6, Grik2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h8i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h8i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4h8i RCSB], [http://www.ebi.ac.uk/pdbsum/4h8i PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4h8i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4h8i OCA], [http://pdbe.org/4h8i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4h8i RCSB], [http://www.ebi.ac.uk/pdbsum/4h8i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4h8i ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4h8i" style="background-color:#fffaf0;"></div>
 ==See Also==

4h8i

From Proteopedia

Revision as of 19:00, 4 August 2016

Structure of GluK2-LBD in complex with GluAzo

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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