1wp9
From Proteopedia
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|PDB= 1wp9 |SIZE=350|CAPTION= <scene name='initialview01'>1wp9</scene>, resolution 2.9Å | |PDB= 1wp9 |SIZE=350|CAPTION= <scene name='initialview01'>1wp9</scene>, resolution 2.9Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=PO4:PHOSPHATE ION'>PO4</scene> | + | |LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Adenosinetriphosphatase Adenosinetriphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.3 3.6.1.3] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Adenosinetriphosphatase Adenosinetriphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.3 3.6.1.3] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1j22|1j22]], [[1j23|1j23]], [[1j24|1j24]], [[1j25|1j25]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wp9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wp9 OCA], [http://www.ebi.ac.uk/pdbsum/1wp9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1wp9 RCSB]</span> | ||
}} | }} | ||
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[[Category: Nishino, T.]] | [[Category: Nishino, T.]] | ||
[[Category: Tsuchiya, D.]] | [[Category: Tsuchiya, D.]] | ||
| - | [[Category: PO4]] | ||
[[Category: atpase]] | [[Category: atpase]] | ||
[[Category: dna recombination]] | [[Category: dna recombination]] | ||
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[[Category: helicase]] | [[Category: helicase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:39:18 2008'' |
Revision as of 21:39, 30 March 2008
| |||||||
| , resolution 2.9Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Activity: | Adenosinetriphosphatase, with EC number 3.6.1.3 | ||||||
| Related: | 1j22, 1j23, 1j24, 1j25
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of Pyrococcus furiosus Hef helicase domain
Overview
DNA and RNA frequently form various branched intermediates that are important for the transmission of genetic information. Helicases play pivotal roles in the processing of these transient intermediates during nucleic acid metabolism. The archaeal Hef helicase/ nuclease is a representative protein that processes flap- or fork-DNA structures, and, intriguingly, its C-terminal half belongs to the XPF/Mus81 nuclease family. Here, we report the crystal structure of the helicase domain of the Hef protein from Pyrococcus furiosus. The structure reveals a novel helical insertion between the two conserved helicase core domains. This positively charged extra region, structurally similar to the "thumb" domain of DNA polymerase, plays critical roles in fork recognition. The Hef helicase/nuclease exhibits sequence similarity to the Mph1 helicase from Saccharomyces cerevisiae; XPF/Rad1, involved in DNA repair; and a putative Hef homolog identified in mammals. Hence, our findings provide a structural basis for the functional mechanisms of this helicase/nuclease family.
About this Structure
1WP9 is a Single protein structure of sequence from Pyrococcus furiosus dsm 3638. Full crystallographic information is available from OCA.
Reference
Crystal structure and functional implications of Pyrococcus furiosus hef helicase domain involved in branched DNA processing., Nishino T, Komori K, Tsuchiya D, Ishino Y, Morikawa K, Structure. 2005 Jan;13(1):143-53. PMID:15642269
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