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1wpx

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|PDB= 1wpx |SIZE=350|CAPTION= <scene name='initialview01'>1wpx</scene>, resolution 2.70&Aring;
|PDB= 1wpx |SIZE=350|CAPTION= <scene name='initialview01'>1wpx</scene>, resolution 2.70&Aring;
|SITE=
|SITE=
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|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=ACE:ACETYL GROUP'>ACE</scene>
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|LIGAND= <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Carboxypeptidase_C Carboxypeptidase C], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.16.5 3.4.16.5]
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Carboxypeptidase_C Carboxypeptidase C], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.16.5 3.4.16.5] </span>
|GENE=
|GENE=
 +
|DOMAIN=
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|RELATEDENTRY=[[1bd9|1BD9]], [[1beh|1BEH]], [[1a44|1A44]], [[1qou|1QOU]], [[1fjj|1FJJ]], [[1fux|1FUX]]
 +
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wpx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wpx OCA], [http://www.ebi.ac.uk/pdbsum/1wpx PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1wpx RCSB]</span>
}}
}}
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[[Category: Narita, Y.]]
[[Category: Narita, Y.]]
[[Category: Ueda, M.]]
[[Category: Ueda, M.]]
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[[Category: ACE]]
 
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[[Category: NAG]]
 
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[[Category: NDG]]
 
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[[Category: SO4]]
 
[[Category: carboxypeptidase inhibitor]]
[[Category: carboxypeptidase inhibitor]]
[[Category: phosphatidylethanolamine-binding protein]]
[[Category: phosphatidylethanolamine-binding protein]]
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[[Category: serine proteinase inhibitor]]
[[Category: serine proteinase inhibitor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 15:00:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:39:39 2008''

Revision as of 21:39, 30 March 2008

Image:1wpx.gif


PDB ID 1wpx

Drag the structure with the mouse to rotate
, resolution 2.70Å
Ligands: , , ,
Activity: Carboxypeptidase C, with EC number 3.4.16.5
Related: 1BD9, 1BEH, 1A44, 1QOU, 1FJJ, 1FUX


Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Crystal structure of carboxypeptidase Y inhibitor complexed with the cognate proteinase


Overview

Carboxypeptidase Y (CPY) inhibitor, IC, shows no homology to any other known proteinase inhibitors and rather belongs to the phosphatidylethanolamine-binding protein (PEBP) family. We report here on the crystal structure of the IC-CPY complex at 2.7 A resolution. The structure of IC in the complex with CPY consists of one major beta-type domain and a N-terminal helical segment. The structure of the complex contains two binding sites of IC toward CPY, the N-terminal inhibitory reactive site (the primary CPY-binding site) and the secondary CPY-binding site, which interact with the S1 substrate-binding site of CPY and the hydrophobic surface flanked by the active site of the enzyme, respectively. It was also revealed that IC had the ligand-binding site, which is conserved among PEBPs and the putative binding site of the polar head group of phospholipid. The complex structure and analyses of IC mutants for inhibitory activity and the binding to CPY demonstrate that the N-terminal inhibitory reactive site is essential both for inhibitory function and the complex formation with CPY and that the binding of IC to CPY constitutes a novel mode of the proteinase-protein inhibitor interaction. The unique binding mode of IC toward the cognate proteinase provides insights into the inhibitory mechanism of PEBPs toward serine proteinases and into the specific biological functions of IC belonging to the PEBP family as well.

About this Structure

1WPX is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Structure of the carboxypeptidase Y inhibitor IC in complex with the cognate proteinase reveals a novel mode of the proteinase-protein inhibitor interaction., Mima J, Hayashida M, Fujii T, Narita Y, Hayashi R, Ueda M, Hata Y, J Mol Biol. 2005 Mar 11;346(5):1323-34. Epub 2005 Jan 25. PMID:15713484

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