1cc3

From Proteopedia

Revision as of 06:44, 30 October 2007

PURPLE CUA CENTER

Overview

The X-ray structure of an engineered purple CuA center in azurin from, Pseudomonas aeruginosa has been determined and refined at 1.65 A, resolution. Two independent purple CuA azurin molecules are in the, asymmetric unit of a new P21 crystal, and they have nearly identical, conformations (rmsd of 0.27 A for backbone atoms). The purple CuA azurin, was produced by the loop-engineering strategy, and the resulting overall, structure is unperturbed. The insertion of a slightly larger Cu-binding, loop into azurin causes the two structural domains of azurin to move away, from each other. The high-resolution structure reveals the detailed, environment of the delocalized mixed-valence [Cu(1.5).Cu(1.5)] binuclear, purple CuA center, which serves as a useful reference model for other, native ... [(full description)]

About this Structure

1CC3 is a [Single protein] structure of sequence from [Pseudomonas aeruginosa] with CU as [ligand]. Structure known Active Sites: CUA and CUB. Full crystallographic information is available from [OCA].

Reference

Structural basis of electron transfer modulation in the purple CuA center., Robinson H, Ang MC, Gao YG, Hay MT, Lu Y, Wang AH, Biochemistry. 1999 May 4;38(18):5677-83. PMID:10231517

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