1wu4
From Proteopedia
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|PDB= 1wu4 |SIZE=350|CAPTION= <scene name='initialview01'>1wu4</scene>, resolution 1.35Å | |PDB= 1wu4 |SIZE=350|CAPTION= <scene name='initialview01'>1wu4</scene>, resolution 1.35Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Oligosaccharide_reducing-end_xylanase Oligosaccharide reducing-end xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.156 3.2.1.156] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Oligosaccharide_reducing-end_xylanase Oligosaccharide reducing-end xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.156 3.2.1.156] </span> |
|GENE= BH2105 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2 Bacteria]) | |GENE= BH2105 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2 Bacteria]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1wu5|1WU5]], [[1wu6|1WU6]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wu4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wu4 OCA], [http://www.ebi.ac.uk/pdbsum/1wu4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1wu4 RCSB]</span> | ||
}} | }} | ||
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[[Category: Shoun, H.]] | [[Category: Shoun, H.]] | ||
[[Category: Wakagi, T.]] | [[Category: Wakagi, T.]] | ||
| - | [[Category: GOL]] | ||
| - | [[Category: NI]] | ||
[[Category: (alpla/alpha)6 barrel]] | [[Category: (alpla/alpha)6 barrel]] | ||
[[Category: glycoside hydrolase family 8]] | [[Category: glycoside hydrolase family 8]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:41:04 2008'' |
Revision as of 21:41, 30 March 2008
| |||||||
| , resolution 1.35Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | BH2105 (Bacteria) | ||||||
| Activity: | Oligosaccharide reducing-end xylanase, with EC number 3.2.1.156 | ||||||
| Related: | 1WU5, 1WU6
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of reducing-end-xylose releasing exo-oligoxylanase
Overview
Reducing end xylose-releasing exo-oligoxylanase from Bacillus halodurans C-125 (Rex) hydrolyzes xylooligosaccharides whose degree of polymerization is greater than or equal to 3, releasing the xylose unit at the reducing end. It is a unique exo-type glycoside hydrolase that recognizes the xylose unit at the reducing end in a very strict manner, even discriminating the beta-anomeric hydroxyl configuration from the alpha-anomer or 1-deoxyxylose. We have determined the crystal structures of Rex in unliganded and complex forms at 1.35-2.20-A resolution and revealed the structural aspects of its three subsites ranging from -2 to +1. The structure of Rex was compared with those of endo-type enzymes in glycoside hydrolase subfamily 8a (GH-8a). The catalytic machinery of Rex is basically conserved with other GH-8a enzymes. However, subsite +2 is blocked by a barrier formed by a kink in the loop before helix alpha10. His-319 in this loop forms a direct hydrogen bond with the beta-hydroxyl of xylose at subsite +1, contributing to the specific recognition of anomers at the reducing end.
About this Structure
1WU4 is a Single protein structure of sequence from Bacteria. Full crystallographic information is available from OCA.
Reference
Structural basis for the specificity of the reducing end xylose-releasing exo-oligoxylanase from Bacillus halodurans C-125., Fushinobu S, Hidaka M, Honda Y, Wakagi T, Shoun H, Kitaoka M, J Biol Chem. 2005 Apr 29;280(17):17180-6. Epub 2005 Feb 17. PMID:15718242
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