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1smt

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Revision as of 00:33, 10 September 2015

SMTB REPRESSOR FROM SYNECHOCOCCUS PCC7942

Structural highlights

1smt is a 2 chain structure with sequence from Anacystis nidulans r2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum

Function

[SMTB_SYNE7] Transcriptional repressor of the expression of the smtA gene. Binds two zinc ions per dimer. The complex of DNA and SmtB is dissociated by zinc ions.^[1] ^[2] ^[3]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

SmtB from Synechococcus PCC7942 is a trans-acting dimeric repressor that is required for Zn(2+)-responsive expression of the metallothionein SmtA. The structure of SmtB was solved using multiple isomorphous replacement techniques and refined at 2.2 A resolution by simulated annealing to an R-factor of 0.218. SmtB displays the classical helix-turn-helix motif found in many DNA-binding proteins. It has an alpha + beta topology, and the arrangement of the three core helices and the beta hairpin is similar to the HNF-3/fork head, CAP and diphtheria toxin repressor proteins. Although there is no zinc in the crystal structure, analysis of a mercuric acetate derivative suggests a total of four Zn2+ binding sites in the dimer. Two of these putative sites are at the opposite ends of the dimer, while the other two are at the dimer interface and are formed by residues contributed from each monomer. The structure of the dimer is such that simultaneous binding for both recognition helices to DNA would require either a bend in the DNA helix or a conformational change in the dimer. The structure of Synechococcus SmtB is the first in this family of metal-binding DNA repressors.

Crystal structure of the cyanobacterial metallothionein repressor SmtB: a model for metalloregulatory proteins.,Cook WJ, Kar SR, Taylor KB, Hall LM J Mol Biol. 1998 Jan 16;275(2):337-46. PMID:9466913^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ VanZile ML, Chen X, Giedroc DP. Allosteric negative regulation of smt O/P binding of the zinc sensor, SmtB, by metal ions: a coupled equilibrium analysis. Biochemistry. 2002 Aug 6;41(31):9776-86. PMID:12146943
↑ Morita EH, Wakamatsu M, Uegaki K, Yumoto N, Kyogoku Y, Hayashi H. Zinc ions inhibit the protein-DNA complex formation between cyanobacterial transcription factor SmtB and its recognition DNA sequences. Plant Cell Physiol. 2002 Oct;43(10):1254-8. PMID:12407207
↑ Turner JS, Glands PD, Samson AC, Robinson NJ. Zn2+-sensing by the cyanobacterial metallothionein repressor SmtB: different motifs mediate metal-induced protein-DNA dissociation. Nucleic Acids Res. 1996 Oct 1;24(19):3714-21. PMID:8871549
↑ Cook WJ, Kar SR, Taylor KB, Hall LM. Crystal structure of the cyanobacterial metallothionein repressor SmtB: a model for metalloregulatory proteins. J Mol Biol. 1998 Jan 16;275(2):337-46. PMID:9466913 doi:10.1006/jmbi.1997.1443

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1smt"

Categories: Anacystis nidulans r2 | Cook, W J | Hall, L M | Dna repressor | Metalloregulation

@@ Line 2: / Line 2: @@
 <StructureSection load='1smt' size='340' side='right' caption='[[1smt]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1smt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Synechococcus_elongatus Synechococcus elongatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SMT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SMT FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1smt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Anacystis_nidulans_r2 Anacystis nidulans r2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SMT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SMT FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1smt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1smt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1smt RCSB], [http://www.ebi.ac.uk/pdbsum/1smt PDBsum]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1smt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1smt OCA], [http://pdbe.org/1smt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1smt RCSB], [http://www.ebi.ac.uk/pdbsum/1smt PDBsum]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/SMTB_SYNP7 SMTB_SYNP7]] Transcriptional repressor of the expression of the smtA gene. Binds two zinc ions per dimer. The complex of DNA and SmtB is dissociated by zinc ions.<ref>PMID:8871549</ref> <ref>PMID:12146943</ref> <ref>PMID:12407207</ref>
+[[http://www.uniprot.org/uniprot/SMTB_SYNE7 SMTB_SYNE7]] Transcriptional repressor of the expression of the smtA gene. Binds two zinc ions per dimer. The complex of DNA and SmtB is dissociated by zinc ions.<ref>PMID:12146943</ref> <ref>PMID:12407207</ref> <ref>PMID:8871549</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 25: / Line 25: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 1smt" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Synechococcus elongatus]]
+[[Category: Anacystis nidulans r2]]
 [[Category: Cook, W J]]
 [[Category: Hall, L M]]
 [[Category: Dna repressor]]
 [[Category: Metalloregulation]]

1smt

From Proteopedia

Revision as of 00:33, 10 September 2015

SMTB REPRESSOR FROM SYNECHOCOCCUS PCC7942

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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