1x8z
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= AT1G48020 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana]) | |GENE= AT1G48020 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 Arabidopsis thaliana]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1rj1|1RJ1]], [[1rj4|1RJ4]], [[1x90|1X90]], [[1x91|1X91]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x8z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x8z OCA], [http://www.ebi.ac.uk/pdbsum/1x8z PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1x8z RCSB]</span> | ||
}} | }} | ||
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[[Category: four-helix bundle]] | [[Category: four-helix bundle]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:46:25 2008'' |
Revision as of 21:46, 30 March 2008
| |||||||
| , resolution 2.86Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | AT1G48020 (Arabidopsis thaliana) | ||||||
| Related: | 1RJ1, 1RJ4, 1X90, 1X91
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of a pectin methylesterase inhibitor from Arabidopsis thaliana
Overview
Pectin methylesterase (PME) and invertase are key enzymes in plant carbohydrate metabolism. Inhibitors of both enzymes constitute a sequence family of extracellular proteins. Members of this family are selectively targeted toward either PME or invertase. In a comparative structural approach we have studied how this target specificity is implemented on homologous sequences. By extending crystallographic work on the invertase inhibitor Nt-CIF to a pectin methylesterase inhibitor (PMEI) from Arabidopsis thaliana, we show an alpha-helical hairpin motif to be an independent and mobile structural entity in PMEI. Removal of this hairpin fully inactivates the inhibitor. A chimera composed of the alpha-hairpin of PMEI and the four-helix bundle of Nt-CIF is still active against PME. By contrast, combining the corresponding segment of Nt-CIF with the four-helix bundle of PMEI renders the protein inactive toward either PME or invertase. Our experiments provide insight in how these homologous inhibitors can make differential use of similar structural modules to achieve distinct functions. Integrating our results with previous findings, we present a model for the PME-PMEI complex with important implications.
About this Structure
1X8Z is a Single protein structure of sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA.
Reference
Structural insights into the target specificity of plant invertase and pectin methylesterase inhibitory proteins., Hothorn M, Wolf S, Aloy P, Greiner S, Scheffzek K, Plant Cell. 2004 Dec;16(12):3437-47. Epub 2004 Nov 4. PMID:15528298
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