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4pcw
From Proteopedia
(Difference between revisions)
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| - | ''' | + | ==Crystal Structure of the N-terminal Domain of Human Profilaggrin at 2.2 A Resolution== |
| + | <StructureSection load='4pcw' size='340' side='right' caption='[[4pcw]], [[Resolution|resolution]] 2.20Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4pcw]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PCW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PCW FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2PE:NONAETHYLENE+GLYCOL'>2PE</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pcw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pcw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4pcw RCSB], [http://www.ebi.ac.uk/pdbsum/4pcw PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | == Disease == | ||
| + | [[http://www.uniprot.org/uniprot/FILA_HUMAN FILA_HUMAN]] Autosomal dominant ichthyosis vulgaris. The disease is caused by mutations affecting the gene represented in this entry. Disease susceptibility is associated with variations affecting the gene represented in this entry. | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/FILA_HUMAN FILA_HUMAN]] Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The fused-type S100 protein profilaggrin and its proteolytic products including filaggrin are important in the formation of a normal epidermal barrier; however, the specific function of the S100 calcium-binding domain in profilaggrin biology is poorly understood. To explore its molecular function, we determined a 2.2 A-resolution crystal structure of the N-terminal fused-type S100 domain of human profilaggrin with bound calcium ions. The profilaggrin S100 domain formed a stable dimer, which contained two hydrophobic pockets that provide a molecular interface for protein interactions. Biochemical and molecular approaches demonstrated that three proteins, annexin II/p36, stratifin/14-3-3 sigma, and Hsp27, bind to the N-terminal domain of human profilaggrin; one protein (stratifin) co-localized with profilaggrin in the differentiating granular cell layer of human skin. Together, these findings suggest a model where the profilaggrin N-terminus uses calcium-dependent and calcium-independent protein-protein interactions to regulate its involvement in keratinocyte terminal differentiation and incorporation into the cornified cell envelope.Journal of Investigative Dermatology accepted article preview online, 11 March 2015. doi:10.1038/jid.2015.102. | ||
| - | + | Crystal Structure of Human Profilaggrin S100 Domain and Identification of Target Proteins Annexin II, Stratifin and hsp27.,Bunick CG, Presland RB, Lawrence OT, Pearton DJ, Milstone LM, Steitz TA J Invest Dermatol. 2015 Mar 11. doi: 10.1038/jid.2015.102. PMID:25760235<ref>PMID:25760235</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| + | [[Category: Bunick, C G]] | ||
| + | [[Category: Steitz, T A]] | ||
| + | [[Category: Ef-hand calcium binding protein]] | ||
| + | [[Category: Epidermal skin protein]] | ||
| + | [[Category: Metal binding protein]] | ||
| + | [[Category: S100 protein]] | ||
| + | [[Category: Signaling protein]] | ||
Revision as of 13:16, 1 April 2015
Crystal Structure of the N-terminal Domain of Human Profilaggrin at 2.2 A Resolution
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