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1xjv
From Proteopedia
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|PDB= 1xjv |SIZE=350|CAPTION= <scene name='initialview01'>1xjv</scene>, resolution 1.73Å | |PDB= 1xjv |SIZE=350|CAPTION= <scene name='initialview01'>1xjv</scene>, resolution 1.73Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= POT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |GENE= POT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xjv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xjv OCA], [http://www.ebi.ac.uk/pdbsum/1xjv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1xjv RCSB]</span> | ||
}} | }} | ||
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[[Category: Lei, M.]] | [[Category: Lei, M.]] | ||
[[Category: Podell, E R.]] | [[Category: Podell, E R.]] | ||
| - | [[Category: | + | [[Category: protein-dna complex]] |
| + | [[Category: single-stranded dna]] | ||
| + | [[Category: telomere]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:50:35 2008'' |
Revision as of 21:50, 30 March 2008
| |||||||
| , resolution 1.73Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Gene: | POT1 (Homo sapiens) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of human POT1 bound to telomeric single-stranded DNA (TTAGGGTTAG)
Overview
The POT1 (protection of telomeres 1) protein binds the single-stranded overhang at the ends of chromosomes in diverse eukaryotes. It is essential for chromosome end-protection in the fission yeast Schizosaccharomyces pombe, and it is involved in regulation of telomere length in human cells. Here, we report the crystal structure at a resolution of 1.73 A of the N-terminal half of human POT1 (hPOT1) protein bound to a telomeric single-stranded DNA (ssDNA) decamer, TTAGGGTTAG, the minimum tight-binding sequence indicated by in vitro binding assays. The structure reveals that hPOT1 contains two oligonucleotide/ oligosaccharide-binding (OB) folds; the N-terminal OB fold binds the first six nucleotides, resembling the structure of the S. pombe Pot1pN-ssDNA complex, whereas the second OB fold binds and protects the 3' end of the ssDNA. These results provide an atomic-resolution model for chromosome end-capping.
About this Structure
1XJV is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of human POT1 bound to telomeric single-stranded DNA provides a model for chromosome end-protection., Lei M, Podell ER, Cech TR, Nat Struct Mol Biol. 2004 Dec;11(12):1223-9. Epub 2004 Nov 21. PMID:15558049
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