1xpu
From Proteopedia
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|PDB= 1xpu |SIZE=350|CAPTION= <scene name='initialview01'>1xpu</scene>, resolution 3.05Å | |PDB= 1xpu |SIZE=350|CAPTION= <scene name='initialview01'>1xpu</scene>, resolution 3.05Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>AGS</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=FPD:5A-(3-FORMYLPHENYLSULFANYL)-DIHYDROBICYCLOMYCIN'>FPD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1xpo|1XPO]], [[1xpr|1XPR]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xpu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xpu OCA], [http://www.ebi.ac.uk/pdbsum/1xpu PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1xpu RCSB]</span> | ||
}} | }} | ||
| Line 27: | Line 30: | ||
[[Category: Park, B S.]] | [[Category: Park, B S.]] | ||
[[Category: Skordalakes, E.]] | [[Category: Skordalakes, E.]] | ||
| - | [[Category: | + | [[Category: 5a-(3-formylphenylsulfanyl)-dihydrobicyclomycin (fpdb)]] |
| - | [[Category: | + | [[Category: atpgamma]] |
| - | + | [[Category: rho transcription termination factor]] | |
| - | [[Category: rho transcription termination factor | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:52:56 2008'' |
Revision as of 21:52, 30 March 2008
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| , resolution 3.05Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , | ||||||
| Related: | 1XPO, 1XPR
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structural mechanism of inhibition of the Rho transcription termination factor by the antibiotic 5a-(3-formylphenylsulfanyl)-dihydrobicyclomycin (FPDB)
Overview
Rho is a hexameric RNA/DNA helicase/translocase that terminates transcription of select genes in bacteria. The naturally occurring antibiotic, bicyclomycin (BCM), acts as a noncompetitive inhibitor of ATP turnover to disrupt this process. We have determined three independent X-ray crystal structures of Rho complexed with BCM and two semisynthetic derivatives, 5a-(3-formylphenylsulfanyl)-dihydrobicyclomycin (FPDB) and 5a-formylbicyclomycin (FB) to 3.15, 3.05, and 3.15 A resolution, respectively. The structures show that BCM and its derivatives are nonnucleotide inhibitors that interact with Rho at a pocket adjacent to the ATP and RNA binding sites in the C-terminal half of the protein. BCM association prevents ATP turnover by an unexpected mechanism, occluding the binding of the nucleophilic water molecule required for ATP hydrolysis. Our data explain why only certain elements of BCM have been amenable to modification and serve as a template for the design of new inhibitors.
About this Structure
1XPU is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structural mechanism of inhibition of the Rho transcription termination factor by the antibiotic bicyclomycin., Skordalakes E, Brogan AP, Park BS, Kohn H, Berger JM, Structure. 2005 Jan;13(1):99-109. PMID:15642265
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