1y0q
From Proteopedia
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|PDB= 1y0q |SIZE=350|CAPTION= <scene name='initialview01'>1y0q</scene>, resolution 3.60Å | |PDB= 1y0q |SIZE=350|CAPTION= <scene name='initialview01'>1y0q</scene>, resolution 3.60Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SPK:SPERMINE+(FULLY+PROTONATED+FORM)'>SPK</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= orf142-I2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=55510 Staphylococcus phage Twort]) | |GENE= orf142-I2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=55510 Staphylococcus phage Twort]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1y0q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y0q OCA], [http://www.ebi.ac.uk/pdbsum/1y0q PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1y0q RCSB]</span> | ||
}} | }} | ||
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[[Category: Golden, B L.]] | [[Category: Golden, B L.]] | ||
[[Category: Kim, H.]] | [[Category: Kim, H.]] | ||
| - | [[Category: MG]] | ||
| - | [[Category: SO4]] | ||
| - | [[Category: SPK]] | ||
[[Category: guanosine-binding]] | [[Category: guanosine-binding]] | ||
[[Category: ribozyme]] | [[Category: ribozyme]] | ||
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[[Category: rna-splicing]] | [[Category: rna-splicing]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 00:57:16 2008'' |
Revision as of 21:57, 30 March 2008
| |||||||
| , resolution 3.60Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , , , | ||||||
| Gene: | orf142-I2 (Staphylococcus phage Twort) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of an active group I ribozyme-product complex
Overview
Group I introns are catalytic RNAs capable of orchestrating two sequential phosphotransesterification reactions that result in self-splicing. To understand how the group I intron active site facilitates catalysis, we have solved the structure of an active ribozyme derived from the orf142-I2 intron from phage Twort bound to a four-nucleotide product RNA at a resolution of 3.6 A. In addition to the three conserved domains characteristic of all group I introns, the Twort ribozyme has peripheral insertions characteristic of phage introns. These elements form a ring that completely envelops the active site, where a snug pocket for guanosine is formed by a series of stacked base triples. The structure of the active site reveals three potential binding sites for catalytic metals, and invokes a role for the 2' hydroxyl of the guanosine substrate in organization of the active site for catalysis.
About this Structure
1Y0Q is a Protein complex structure of sequences from Staphylococcus phage twort. Full crystallographic information is available from OCA.
Reference
Crystal structure of a phage Twort group I ribozyme-product complex., Golden BL, Kim H, Chase E, Nat Struct Mol Biol. 2005 Jan;12(1):82-9. Epub 2004 Dec 5. PMID:15580277
Page seeded by OCA on Mon Mar 31 00:57:16 2008
