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4rih
From Proteopedia
(Difference between revisions)
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| - | ''' | + | ==Chimeric Glycosyltransferase LanGT2S8Ac, carbasugar substrate complex== |
| + | <StructureSection load='4rih' size='340' side='right' caption='[[4rih]], [[Resolution|resolution]] 2.22Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4rih]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RIH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RIH FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3R2:2-DEOXY-5-O-[(R)-{[(R)-{[(1S,3R,4R,5S)-3,4-DIHYDROXY-5-METHYLCYCLOHEXYL]OXY}(HYDROXY)PHOSPHORYL]OXY}(HYDROXY)PHOSPHORYL]-3,4-DIHYDROTHYMIDINE'>3R2</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rih FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rih OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rih RCSB], [http://www.ebi.ac.uk/pdbsum/4rih PDBsum]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The structures of the O-glycosyltransferase LanGT2 and the engineered, CC bond-forming variant LanGT2S8Ac show how the replacement of a single loop can change the functionality of the enzyme. Crystal structures of the enzymes in complex with a nonhydrolyzable nucleotide-sugar analogue revealed that there is a conformational transition to create the binding sites for the aglycon substrate. This induced-fit transition was explored by molecular docking experiments with various aglycon substrates. | ||
| - | + | Structural Characterization of O- and C-Glycosylating Variants of the Landomycin Glycosyltransferase LanGT2.,Tam HK, Harle J, Gerhardt S, Rohr J, Wang G, Thorson JS, Bigot A, Lutterbeck M, Seiche W, Breit B, Bechthold A, Einsle O Angew Chem Int Ed Engl. 2015 Jan 7. doi: 10.1002/anie.201409792. PMID:25581707<ref>PMID:25581707</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | + | == References == | |
| - | + | <references/> | |
| - | + | __TOC__ | |
| - | [[Category: | + | </StructureSection> |
| + | [[Category: Bechthold, A]] | ||
[[Category: Breit, B]] | [[Category: Breit, B]] | ||
| - | [[Category: Bechthold, A]] | ||
[[Category: Einsle, O]] | [[Category: Einsle, O]] | ||
| + | [[Category: Gerhardt, S]] | ||
| + | [[Category: Tam, H K]] | ||
| + | [[Category: C-glycosylating glycosyltransferase]] | ||
| + | [[Category: Gt fold]] | ||
| + | [[Category: Transferase]] | ||
Revision as of 16:34, 28 January 2015
Chimeric Glycosyltransferase LanGT2S8Ac, carbasugar substrate complex
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