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3zip

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Revision as of 16:19, 4 August 2016

minor-site specific NLS (A58)

Structural highlights

3zip is a 3 chain structure with sequence from Lk3 transgenic mice and Synthetic construct sequences. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	3zin, 3zio, 3ziq, 3zir
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[IMA2_MOUSE] Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.

Publication Abstract from PubMed

Nuclear localization signals (NLSs) contain one or two clusters of basic residues and are recognized by the import receptor importin-alpha. There are two NLS-binding sites (major and minor) on importin-alpha and the major NLS-binding site is considered to be the primary binding site. Here, we used crystallographic and biochemical methods to investigate the binding between importin-alpha and predicted "minor site-specific" NLSs: four peptide library-derived peptides, and the NLS from mouse RNA helicase II/Gualpha. The crystal structures reveal that these atypical NLSs indeed preferentially bind to the minor NLS-binding site. Unlike previously characterized NLSs, the C-terminal residues of these NLSs form an alpha-helical turn, stabilized by internal H-bond and cation-pi interactions between the aromatic residues from the NLSs and the positively-charged residues from importin-alpha. This helical turn sterically hinders binding at the major NLS-binding site, explaining the minor-site preference. Our data suggest the sequence RXXKR[K/X][F/Y/W]XXAF as the optimal minor NLS-binding site-specific motif, which may help identify novel proteins with atypical NLSs.

Distinctive Conformation of Minor Site-Specific Nuclear Localization Signals Bound to Importin-alpha,Chang CW, Counago RM, Williams SJ, Boden M, Kobe B Traffic. 2013 Aug 2. doi: 10.1111/tra.12098. PMID:23910026^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chang CW, Counago RM, Williams SJ, Boden M, Kobe B. Distinctive Conformation of Minor Site-Specific Nuclear Localization Signals Bound to Importin-alpha Traffic. 2013 Aug 2. doi: 10.1111/tra.12098. PMID:23910026 doi:10.1111/tra.12098

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3zip"

@@ Line 1: / Line 1: @@
 ==minor-site specific NLS (A58)==
 <StructureSection load='3zip' size='340' side='right' caption='[[3zip]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3zip]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZIP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZIP FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3zip]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice] and [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZIP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ZIP FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3zin|3zin]], [[3zio|3zio]], [[3ziq|3ziq]], [[3zir|3zir]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zip OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zip RCSB], [http://www.ebi.ac.uk/pdbsum/3zip PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zip OCA], [http://pdbe.org/3zip PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zip RCSB], [http://www.ebi.ac.uk/pdbsum/3zip PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zip ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 16: / Line 17: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3zip" style="background-color:#fffaf0;"></div>
 ==See Also==
@@ Line 23: / Line 25: @@
 __TOC__
 </StructureSection>
-[[Category: Mus musculus]]
+[[Category: Lk3 transgenic mice]]
-[[Category: Synthetic construct]]
+[[Category: Synthetic construct sequences]]
 [[Category: Chang, C W]]
 [[Category: Counago, R M]]

3zip

From Proteopedia

Revision as of 16:19, 4 August 2016

minor-site specific NLS (A58)

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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