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3zv2

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Revision as of 16:35, 4 August 2016

HUMAN PROTEIN-TYROSINE PHOSPHATASE 1B C215A, S216A MUTANT

Structural highlights

3zv2 is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	2f6t, 2f6f, 2cnf, 2cm3, 1g1f, 2cm2, 1q6n, 1ptu, 1c84, 2veu, 1nz7, 1q1m, 2azr, 1c88, 1oet, 1c83, 1sug, 1onz, 1g1g, 1xbo, 1ptv, 2cnh, 2hnp, 1nwl, 1t49, 2f6y, 2cne, 1nl9, 1c86, 1kav, 2hnq, 1jf7, 1pxh, 1q6s, 1l8g, 2cma, 1q6m, 1bzj, 1i57, 1wax, 1ony, 1g1h, 1kak, 2cng, 1t4j, 2cni, 2b07, 2cm8, 1eeo, 1g7g, 1ptt, 1qxk, 1ecv, 1oem, 1nwe, 2cmb, 2fjn, 1aax, 2vev, 2vey, 2b4s, 2cm7, 2bgd, 1a5y, 2vex, 1c85, 2cmc, 2vew, 1lqf, 2f6w, 1c87, 1no6, 1pyn, 2bge, 1oes, 1bzc, 2f6z, 2f71, 1oeu, 1q6t, 1g7f, 1nny, 1q6j, 2f70, 1q6p, 2f6v, 1t48, 1oeo, 2fjm, 1pa1, 1ph0, 1bzh, 1gfy, 1een, 1pty, 1oev
Activity:	Protein-tyrosine-phosphatase, with EC number 3.1.3.48
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PTN1_HUMAN] Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion.^[1] ^[2]

Publication Abstract from PubMed

Protein tyrosine phosphatase 1B (PTP1B) plays important roles in downregulation of insulin and leptin signaling and is an established therapeutic target for diabetes and obesity. PTP1B is regulated by reactive oxygen species (ROS) produced in response to various stimuli, including insulin. The reversibly oxidized form of the enzyme (PTP1B-OX) is inactive and undergoes profound conformational changes at the active site. We generated conformation-sensor antibodies, in the form of single-chain variable fragments (scFvs), that stabilize PTP1B-OX and thereby inhibit its phosphatase function. Expression of conformation-sensor scFvs as intracellular antibodies (intrabodies) enhanced insulin-induced tyrosyl phosphorylation of the beta subunit of the insulin receptor and its substrate IRS-1 and increased insulin-induced phosphorylation of PKB/AKT. Our data suggest that stabilization of the oxidized, inactive form of PTP1B with appropriate therapeutic molecules may offer a paradigm for phosphatase drug development.

Conformation-Sensing Antibodies Stabilize the Oxidized Form of PTP1B and Inhibit Its Phosphatase Activity.,Haque A, Andersen JN, Salmeen A, Barford D, Tonks NK Cell. 2011 Sep 30;147(1):185-98. PMID:21962515^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Nievergall E, Janes PW, Stegmayer C, Vail ME, Haj FG, Teng SW, Neel BG, Bastiaens PI, Lackmann M. PTP1B regulates Eph receptor function and trafficking. J Cell Biol. 2010 Dec 13;191(6):1189-203. doi: 10.1083/jcb.201005035. Epub 2010, Dec 6. PMID:21135139 doi:10.1083/jcb.201005035
↑ Krishnan N, Fu C, Pappin DJ, Tonks NK. H2S-Induced sulfhydration of the phosphatase PTP1B and its role in the endoplasmic reticulum stress response. Sci Signal. 2011 Dec 13;4(203):ra86. doi: 10.1126/scisignal.2002329. PMID:22169477 doi:10.1126/scisignal.2002329
↑ Haque A, Andersen JN, Salmeen A, Barford D, Tonks NK. Conformation-Sensing Antibodies Stabilize the Oxidized Form of PTP1B and Inhibit Its Phosphatase Activity. Cell. 2011 Sep 30;147(1):185-98. PMID:21962515 doi:10.1016/j.cell.2011.08.036

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3zv2"

@@ Line 1: / Line 1: @@
 ==HUMAN PROTEIN-TYROSINE PHOSPHATASE 1B C215A, S216A MUTANT==
 <StructureSection load='3zv2' size='340' side='right' caption='[[3zv2]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2f6t|2f6t]], [[2f6f|2f6f]], [[2cnf|2cnf]], [[2cm3|2cm3]], [[1g1f|1g1f]], [[2cm2|2cm2]], [[1q6n|1q6n]], [[1ptu|1ptu]], [[1c84|1c84]], [[2veu|2veu]], [[1nz7|1nz7]], [[1q1m|1q1m]], [[2azr|2azr]], [[1c88|1c88]], [[1oet|1oet]], [[1c83|1c83]], [[1sug|1sug]], [[1onz|1onz]], [[1g1g|1g1g]], [[1xbo|1xbo]], [[1ptv|1ptv]], [[2cnh|2cnh]], [[2hnp|2hnp]], [[1nwl|1nwl]], [[1t49|1t49]], [[2f6y|2f6y]], [[2cne|2cne]], [[1nl9|1nl9]], [[1c86|1c86]], [[1kav|1kav]], [[2hnq|2hnq]], [[1jf7|1jf7]], [[1pxh|1pxh]], [[1q6s|1q6s]], [[1l8g|1l8g]], [[2cma|2cma]], [[1q6m|1q6m]], [[1bzj|1bzj]], [[1i57|1i57]], [[1wax|1wax]], [[1ony|1ony]], [[1g1h|1g1h]], [[1kak|1kak]], [[2cng|2cng]], [[1t4j|1t4j]], [[2cni|2cni]], [[2b07|2b07]], [[2cm8|2cm8]], [[1eeo|1eeo]], [[1g7g|1g7g]], [[1ptt|1ptt]], [[1qxk|1qxk]], [[1ecv|1ecv]], [[1oem|1oem]], [[1nwe|1nwe]], [[2cmb|2cmb]], [[2fjn|2fjn]], [[1aax|1aax]], [[2vev|2vev]], [[2vey|2vey]], [[2b4s|2b4s]], [[2cm7|2cm7]], [[2bgd|2bgd]], [[1a5y|1a5y]], [[2vex|2vex]], [[1c85|1c85]], [[2cmc|2cmc]], [[2vew|2vew]], [[1lqf|1lqf]], [[2f6w|2f6w]], [[1c87|1c87]], [[1no6|1no6]], [[1pyn|1pyn]], [[2bge|2bge]], [[1oes|1oes]], [[1bzc|1bzc]], [[2f6z|2f6z]], [[2f71|2f71]], [[1oeu|1oeu]], [[1q6t|1q6t]], [[1g7f|1g7f]], [[1nny|1nny]], [[1q6j|1q6j]], [[2f70|2f70]], [[1q6p|1q6p]], [[2f6v|2f6v]], [[1t48|1t48]], [[1oeo|1oeo]], [[2fjm|2fjm]], [[1pa1|1pa1]], [[1ph0|1ph0]], [[1bzh|1bzh]], [[1gfy|1gfy]], [[1een|1een]], [[1pty|1pty]], [[1oev|1oev]]</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zv2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zv2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3zv2 RCSB], [http://www.ebi.ac.uk/pdbsum/3zv2 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3zv2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zv2 OCA], [http://pdbe.org/3zv2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3zv2 RCSB], [http://www.ebi.ac.uk/pdbsum/3zv2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3zv2 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3zv2" style="background-color:#fffaf0;"></div>
 ==See Also==

3zv2

From Proteopedia

Revision as of 16:35, 4 August 2016

HUMAN PROTEIN-TYROSINE PHOSPHATASE 1B C215A, S216A MUTANT

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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