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2k0a

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Revision as of 04:24, 10 September 2015

1H, 15N and 13C chemical shift assignments for Rds3 protein

Structural highlights

2k0a is a 1 chain structure with sequence from Saccharomyces cerevisiae. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	RDS3 (Saccharomyces cerevisiae)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum

Function

[RDS3_YEAST] Required for pre-mRNA splicing. Involved in regulation of drug sensitivity and may play a role in multidrug resistance.^[1] ^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Rds3p, a component of the U2 snRNP subcomplex SF3b, is essential for pre-mRNA splicing and is extremely well conserved in all eukaryotic species. We report here the solution structure of Rds3p, which reveals an unusual knotted fold unrelated to previously known knotted proteins. Rds3p has a triangular shape with a GATA-like zinc finger at each vertex. Pairs of cysteines contributing to each finger are arranged nonsequentially in a permuted arrangement reminiscent of domain-swapping but which here involves segments of subdomains within a single chain. We suggest that the structure arose through a process of segment swapping after gene duplication. The fingers are connected through beta-strands and loops, forming an overall topology strongly resembling a "triquetra knot." The conservation and surface properties of Rds3p suggest that it functions as a platform for protein assembly within the multiprotein SF3b complex of U2 snRNP. The recombinant protein used for structure determination is biologically active, as it restores splicing activity in a yeast splicing extract depleted of native Rds3p.

Solution structure of the U2 snRNP protein Rds3p reveals a knotted zinc-finger motif.,van Roon AM, Loening NM, Obayashi E, Yang JC, Newman AJ, Hernandez H, Nagai K, Neuhaus D Proc Natl Acad Sci U S A. 2008 Jul 15;105(28):9621-6. Epub 2008 Jul 8. PMID:18621724^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Akache B, Turcotte B. New regulators of drug sensitivity in the family of yeast zinc cluster proteins. J Biol Chem. 2002 Jun 14;277(24):21254-60. Epub 2002 Apr 9. PMID:11943786 doi:http://dx.doi.org/10.1074/jbc.M202566200
↑ Wang Q, Rymond BC. Rds3p is required for stable U2 snRNP recruitment to the splicing apparatus. Mol Cell Biol. 2003 Oct;23(20):7339-49. PMID:14517302
↑ van Roon AM, Loening NM, Obayashi E, Yang JC, Newman AJ, Hernandez H, Nagai K, Neuhaus D. Solution structure of the U2 snRNP protein Rds3p reveals a knotted zinc-finger motif. Proc Natl Acad Sci U S A. 2008 Jul 15;105(28):9621-6. Epub 2008 Jul 8. PMID:18621724

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2k0a"

@@ Line 5: / Line 5: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">RDS3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2k0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k0a OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2k0a RCSB], [http://www.ebi.ac.uk/pdbsum/2k0a PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2k0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k0a OCA], [http://pdbe.org/2k0a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2k0a RCSB], [http://www.ebi.ac.uk/pdbsum/2k0a PDBsum]</span></td></tr>
 </table>
 == Function ==
@@ Line 27: / Line 27: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 2k0a" style="background-color:#fffaf0;"></div>
 ==See Also==

2k0a

From Proteopedia

Revision as of 04:24, 10 September 2015

1H, 15N and 13C chemical shift assignments for Rds3 protein

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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