1yuj
From Proteopedia
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|PDB= 1yuj |SIZE=350|CAPTION= <scene name='initialview01'>1yuj</scene> | |PDB= 1yuj |SIZE=350|CAPTION= <scene name='initialview01'>1yuj</scene> | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | + | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1yui|1YUI]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yuj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yuj OCA], [http://www.ebi.ac.uk/pdbsum/1yuj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1yuj RCSB]</span> | ||
}} | }} | ||
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[[Category: Gronenborn, A M.]] | [[Category: Gronenborn, A M.]] | ||
[[Category: Omichinski, J G.]] | [[Category: Omichinski, J G.]] | ||
| - | [[Category: ZN]] | ||
[[Category: chromatin remodeling]] | [[Category: chromatin remodeling]] | ||
[[Category: complex (dna-binding protein/dna)]] | [[Category: complex (dna-binding protein/dna)]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:23:05 2008'' |
Revision as of 22:23, 30 March 2008
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| Ligands: | , , , , | ||||||
| Related: | 1YUI
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
SOLUTION NMR STRUCTURE OF THE GAGA FACTOR/DNA COMPLEX, 50 STRUCTURES
Overview
The structure of a complex between the DNA binding domain of the GAGA factor (GAGA-DBD) and an oligonucleotide containing its GAGAG consensus binding site has been determined by nuclear magnetic resonance spectroscopy. The GAGA-DBD comprises a single classical Cys2-His2 zinc finger core, and an N-terminal extension containing two highly basic regions, BR1 and BR2. The zinc finger core binds in the major groove and recognizes the first three GAG bases of the consensus in a manner similar to that seen in other classical zinc finger-DNA complexes. Unlike the latter, which require tandem zinc finger repeats with a minimum of two units for high affinity binding, the GAGA-DBD makes use of only a single finger complemented by BR1 and BR2. BR2 forms a helix that interacts in the major groove recognizing the last G of the consensus, while BR1 wraps around the DNA in the minor groove and recognizes the A in the fourth position of the consensus. The implications of the structure of the GAGA-DBD-DNA complex for chromatin remodelling are discussed.
About this Structure
1YUJ is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
The solution structure of a specific GAGA factor-DNA complex reveals a modular binding mode., Omichinski JG, Pedone PV, Felsenfeld G, Gronenborn AM, Clore GM, Nat Struct Biol. 1997 Feb;4(2):122-32. PMID:9033593
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