1yvu
From Proteopedia
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|PDB= 1yvu |SIZE=350|CAPTION= <scene name='initialview01'>1yvu</scene>, resolution 2.90Å | |PDB= 1yvu |SIZE=350|CAPTION= <scene name='initialview01'>1yvu</scene>, resolution 2.90Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | + | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yvu OCA], [http://www.ebi.ac.uk/pdbsum/1yvu PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1yvu RCSB]</span> | ||
}} | }} | ||
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[[Category: Yuan, Y R.]] | [[Category: Yuan, Y R.]] | ||
[[Category: Zhadina, M.]] | [[Category: Zhadina, M.]] | ||
| - | [[Category: CA]] | ||
[[Category: rnase h fold]] | [[Category: rnase h fold]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:24:26 2008'' |
Revision as of 22:24, 30 March 2008
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| , resolution 2.90Å | |||||||
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| Ligands: | , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of A. aeolicus Argonaute
Overview
Argonaute (Ago) proteins constitute a key component of the RNA-induced silencing complex (RISC). We report the crystal structure of Aquifex aeolicus Ago (Aa-Ago) together with binding and cleavage studies, which establish this eubacterial Ago as a bona fide guide DNA strand-mediated site-specific RNA endonuclease. We have generated a stereochemically robust model of the complex, where the guide DNA-mRNA duplex is positioned within a basic channel spanning the bilobal interface, such that the 5' phosphate of the guide strand can be anchored in a basic pocket, and the mRNA can be positioned for site-specific cleavage by RNase H-type divalent cation-coordinated catalytic Asp residues of the PIWI domain. Domain swap experiments involving chimeras of human Ago (hAgo1) and cleavage-competent hAgo2 reinforce the role of the PIWI domain in "slicer" activity. We propose a four-step Ago-mediated catalytic cleavage cycle model, which provides distinct perspectives into the mechanism of guide strand-mediated mRNA cleavage within the RISC.
About this Structure
1YVU is a Single protein structure of sequence from Aquifex aeolicus vf5. Full crystallographic information is available from OCA.
Reference
Crystal structure of A. aeolicus argonaute, a site-specific DNA-guided endoribonuclease, provides insights into RISC-mediated mRNA cleavage., Yuan YR, Pei Y, Ma JB, Kuryavyi V, Zhadina M, Meister G, Chen HY, Dauter Z, Tuschl T, Patel DJ, Mol Cell. 2005 Aug 5;19(3):405-19. PMID:16061186
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