1z5y
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 1z5y |SIZE=350|CAPTION= <scene name='initialview01'>1z5y</scene>, resolution 1.94Å | |PDB= 1z5y |SIZE=350|CAPTION= <scene name='initialview01'>1z5y</scene>, resolution 1.94Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene> | + | |LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Protein-disulfide_reductase Protein-disulfide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.1.8 1.8.1.8] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-disulfide_reductase Protein-disulfide reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.8.1.8 1.8.1.8] </span> |
|GENE= DSBD, DIPZ, CYCZ, CUTA2, B4136 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), DSBE, CCMG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |GENE= DSBD, DIPZ, CYCZ, CUTA2, B4136 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), DSBE, CCMG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1se1|1SE1]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z5y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z5y OCA], [http://www.ebi.ac.uk/pdbsum/1z5y PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1z5y RCSB]</span> | ||
}} | }} | ||
| Line 29: | Line 32: | ||
[[Category: Rozhkova, A.]] | [[Category: Rozhkova, A.]] | ||
[[Category: Stirnimann, C U.]] | [[Category: Stirnimann, C U.]] | ||
| - | [[Category: CL]] | ||
| - | [[Category: EDO]] | ||
[[Category: ccmg]] | [[Category: ccmg]] | ||
[[Category: disulfide-linked]] | [[Category: disulfide-linked]] | ||
| Line 38: | Line 39: | ||
[[Category: thioredoxin-like]] | [[Category: thioredoxin-like]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:29:37 2008'' |
Revision as of 22:29, 30 March 2008
| |||||||
| , resolution 1.94Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | DSBD, DIPZ, CYCZ, CUTA2, B4136 (Escherichia coli), DSBE, CCMG (Escherichia coli) | ||||||
| Activity: | Protein-disulfide reductase, with EC number 1.8.1.8 | ||||||
| Related: | 1SE1
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure Of The Disulfide-Linked Complex Between The N-Terminal Domain Of The Electron Transfer Catalyst DsbD and The Cytochrome c Biogenesis Protein CcmG
Overview
DsbD from Escherichia coli transports two electrons from cytoplasmic thioredoxin to the periplasmic substrate proteins DsbC, DsbG and CcmG. DsbD consists of an N-terminal periplasmic domain (nDsbD), a C-terminal periplasmic domain, and a central transmembrane domain. Each domain possesses two cysteines required for electron transport. Herein, we demonstrate fast (3.9 x 10(5) M(-1)s(-1)) and direct disulfide exchange between nDsbD and CcmG, a highly specific disulfide reductase essential for cytochrome c maturation. We determined the crystal structure of the disulfide-linked complex between nDsbD and the soluble part of CcmG at 1.94 A resolution. In contrast to the other two known complexes of nDsbD with target proteins, the N-terminal segment of nDsbD contributes to specific recognition of CcmG. This and other features, like the possibility of using an additional interaction surface, constitute the structural basis for the adaptability of nDsbD to different protein substrates.
About this Structure
1Z5Y is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structural basis and kinetics of DsbD-dependent cytochrome c maturation., Stirnimann CU, Rozhkova A, Grauschopf U, Grutter MG, Glockshuber R, Capitani G, Structure. 2005 Jul;13(7):985-93. PMID:16004871
Page seeded by OCA on Mon Mar 31 01:29:37 2008
