1zc3
From Proteopedia
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|PDB= 1zc3 |SIZE=350|CAPTION= <scene name='initialview01'>1zc3</scene>, resolution 2.00Å | |PDB= 1zc3 |SIZE=350|CAPTION= <scene name='initialview01'>1zc3</scene>, resolution 2.00Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= RALA, RAL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |GENE= RALA, RAL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1zc4|1ZC4]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zc3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zc3 OCA], [http://www.ebi.ac.uk/pdbsum/1zc3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zc3 RCSB]</span> | ||
}} | }} | ||
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[[Category: Matern, H T.]] | [[Category: Matern, H T.]] | ||
[[Category: Scheller, R H.]] | [[Category: Scheller, R H.]] | ||
| - | [[Category: GNP]] | ||
| - | [[Category: MG]] | ||
[[Category: exocytosis]] | [[Category: exocytosis]] | ||
| - | [[Category: gtp-binding protein]] | + | [[Category: gtp-binding protein,]] |
[[Category: small gtpase]] | [[Category: small gtpase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:33:48 2008'' |
Revision as of 22:33, 30 March 2008
| |||||||
| , resolution 2.00Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | RALA, RAL (Homo sapiens) | ||||||
| Related: | 1ZC4
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the Ral-binding domain of Exo84 in complex with the active RalA
Overview
The Sec6/8 complex, also known as the exocyst complex, is an octameric protein complex that has been implicated in tethering of secretory vesicles to specific regions on the plasma membrane. Two subunits of the Sec6/8 complex, Exo84 and Sec5, have recently been shown to be effector targets for active Ral GTPases. However, the mechanism by which Ral proteins regulate the Sec6/8 activities remains unclear. Here, we present the crystal structure of the Ral-binding domain of Exo84 in complex with active RalA. The structure reveals that the Exo84 Ral-binding domain adopts a pleckstrin homology domain fold, and that RalA interacts with Exo84 via an extended interface that includes both switch regions. Key residues of Exo84 and RalA were found that determine the specificity of the complex interactions; these interactions were confirmed by mutagenesis binding studies. Structural and biochemical data show that Exo84 and Sec5 competitively bind to active RalA. Taken together, these results further strengthen the proposed role of RalA-regulated assembly of the Sec6/8 complex.
About this Structure
1ZC3 is a Protein complex structure of sequences from Homo sapiens and Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Exo84 and Sec5 are competitive regulatory Sec6/8 effectors to the RalA GTPase., Jin R, Junutula JR, Matern HT, Ervin KE, Scheller RH, Brunger AT, EMBO J. 2005 Jun 15;24(12):2064-74. Epub 2005 May 26. PMID:15920473
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