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1zie
From Proteopedia
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|PDB= 1zie |SIZE=350|CAPTION= <scene name='initialview01'>1zie</scene>, resolution 1.44Å | |PDB= 1zie |SIZE=350|CAPTION= <scene name='initialview01'>1zie</scene>, resolution 1.44Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene> | + | |LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=DOD:DEUTERATED+WATER'>DOD</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1a5d|1A5D]], [[1zgt|1ZGT]], [[1ziq|1ZIQ]], [[1zir|1ZIR]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zie FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zie OCA], [http://www.ebi.ac.uk/pdbsum/1zie PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zie RCSB]</span> | ||
}} | }} | ||
| Line 26: | Line 29: | ||
[[Category: McSweeney, S.]] | [[Category: McSweeney, S.]] | ||
[[Category: Timmins, P.]] | [[Category: Timmins, P.]] | ||
| - | [[Category: ACT]] | ||
| - | [[Category: DOD]] | ||
[[Category: 4 greek key motif]] | [[Category: 4 greek key motif]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:37:07 2008'' |
Revision as of 22:37, 30 March 2008
| |||||||
| , resolution 1.44Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Related: | 1A5D, 1ZGT, 1ZIQ, 1ZIR
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Hydrogenated gammaE crystallin in D2O solvent
Overview
Rat gammaE-crystallin was overexpressed, purified under different labelling conditions and crystallized and X-ray data were collected at resolutions between 1.71 and 1.36 A. The structures were determined by molecular replacement. In these structures, the cd loop of the Greek-key motif 3, which is the major structural key motif of the two phase-transition groups of gamma-crystallins, presents a double conformation. The influence of the perdeuteration on the protein structure was determined by comparison of the atomic positions and temperature factors of the different models. The perdeuterated proteins have a similar structure to their hydrogenated counterparts, but partial or full deuteration may have some effect on the atomic B-factor values.
About this Structure
1ZIE is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
A comparison of refined X-ray structures of hydrogenated and perdeuterated rat gammaE-crystallin in H2O and D2O., Artero JB, Hartlein M, McSweeney S, Timmins P, Acta Crystallogr D Biol Crystallogr. 2005 Nov;61(Pt 11):1541-9. Epub 2005, Oct 19. PMID:16239733
Page seeded by OCA on Mon Mar 31 01:37:07 2008
