4x2z
From Proteopedia
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| - | ''' | + | ==Structural view and substrate specificity of papain-like protease from Avian Infectious Bronchitis Virus== |
| - | + | <StructureSection load='4x2z' size='340' side='right' caption='[[4x2z]], [[Resolution|resolution]] 2.15Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[4x2z]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X2Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4X2Z FirstGlance]. <br> | |
| - | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |
| - | + | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4x2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x2z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4x2z RCSB], [http://www.ebi.ac.uk/pdbsum/4x2z PDBsum]</span></td></tr> | |
| - | [[ | + | </table> |
| - | [[ | + | == Function == |
| - | [[ | + | [[http://www.uniprot.org/uniprot/R1AB_IBVB R1AB_IBVB]] The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.<ref>PMID:11413307</ref> The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. Activity of PL-PRO is dependent on zinc (By similarity).<ref>PMID:11413307</ref> The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. Inhibited by the substrate-analog Cbz-Val-Asn-Ser-Thr-Leu-Gln-CMK (By similarity).<ref>PMID:11413307</ref> The peptide p16 might be involved in the EGF signaling pathway.<ref>PMID:11413307</ref> The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Its ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G) (By similarity).<ref>PMID:11413307</ref> The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction (By similarity).<ref>PMID:11413307</ref> Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter (By similarity).<ref>PMID:11413307</ref> Nsp9 is a ssRNA-binding protein (By similarity).<ref>PMID:11413307</ref> NendoU is a Mn(2+)-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond (By similarity).<ref>PMID:11413307</ref> |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
[[Category: Kong, L]] | [[Category: Kong, L]] | ||
| - | [[Category: Yan, L]] | ||
[[Category: Lou, Z]] | [[Category: Lou, Z]] | ||
| + | [[Category: Rao, Z]] | ||
| + | [[Category: Shaw, N]] | ||
| + | [[Category: Yan, L]] | ||
| + | [[Category: Coronavirus]] | ||
| + | [[Category: De-ubiquitination]] | ||
| + | [[Category: Hydrolase]] | ||
| + | [[Category: Ibv]] | ||
| + | [[Category: Plpro]] | ||
Revision as of 16:49, 28 January 2015
Structural view and substrate specificity of papain-like protease from Avian Infectious Bronchitis Virus
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Categories: Kong, L | Lou, Z | Rao, Z | Shaw, N | Yan, L | Coronavirus | De-ubiquitination | Hydrolase | Ibv | Plpro
