1zq2
From Proteopedia
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|PDB= 1zq2 |SIZE=350|CAPTION= <scene name='initialview01'>1zq2</scene>, resolution 1.95Å | |PDB= 1zq2 |SIZE=350|CAPTION= <scene name='initialview01'>1zq2</scene>, resolution 1.95Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=CP:PHOSPHORIC+ACID+MONO(FORMAMIDE)ESTER'>CP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Ornithine_carbamoyltransferase Ornithine carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.3 2.1.3.3] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ornithine_carbamoyltransferase Ornithine carbamoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.3.3 2.1.3.3] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1yh0|1YH0]], [[1yh1|1YH1]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zq2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zq2 OCA], [http://www.ebi.ac.uk/pdbsum/1zq2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zq2 RCSB]</span> | ||
}} | }} | ||
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[[Category: Tuchman, M.]] | [[Category: Tuchman, M.]] | ||
[[Category: Yu, X.]] | [[Category: Yu, X.]] | ||
| - | [[Category: CP]] | ||
| - | [[Category: GOL]] | ||
| - | [[Category: SO4]] | ||
[[Category: alpha/beta two-domain]] | [[Category: alpha/beta two-domain]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:40:09 2008'' |
Revision as of 22:40, 30 March 2008
| |||||||
| , resolution 1.95Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Activity: | Ornithine carbamoyltransferase, with EC number 2.1.3.3 | ||||||
| Related: | 1YH0, 1YH1
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of N-acetyl-L-ornithine transcarbamylase complexed with CP
Overview
N-acetyl-L-ornithine transcarbamoylase (AOTCase) is a new member of the transcarbamoylase superfamily that is essential for arginine biosynthesis in several eubacteria. We report here crystal structures of the binary complexes of AOTCase with its substrates, carbamoyl phosphate (CP) or N-acetyl-L-ornithine (AORN), and the ternary complex with CP and N-acetyl-L-norvaline. Comparison of these structures demonstrates that the substrate-binding mechanism of this novel transcarbamoylase is different from those of aspartate and ornithine transcarbamoylases, both of which show ordered substrate binding with large domain movements. CP and AORN bind to AOTCase independently, and the main conformational change upon substrate binding is ordering of the 80's loop, with a small domain closure around the active site and little movement of the 240's loop. The structures of the complexes provide insight into the mode of substrate binding and the mechanism of the transcarbamoylation reaction.
About this Structure
1ZQ2 is a Single protein structure of sequence from Xanthomonas campestris. Full crystallographic information is available from OCA.
Reference
Structures of N-acetylornithine transcarbamoylase from Xanthomonas campestris complexed with substrates and substrate analogs imply mechanisms for substrate binding and catalysis., Shi D, Yu X, Roth L, Morizono H, Tuchman M, Allewell NM, Proteins. 2006 Aug 1;64(2):532-42. PMID:16741992
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