1zuf
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1d6b|1D6B]], [[1zue|1ZUE]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zuf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zuf OCA], [http://www.ebi.ac.uk/pdbsum/1zuf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zuf RCSB]</span> | ||
}} | }} | ||
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[[Category: helix]] | [[Category: helix]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:41:47 2008'' |
Revision as of 22:41, 30 March 2008
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| Related: | 1D6B, 1ZUE
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution Structure of DLP-4
Overview
The recent discovery that the natriuretic peptide OvCNPb (Ornithorhynchus venom C-type natriuretic peptide B) from platypus (Ornithorynchus anatinus) venom contains a D-amino acid residue suggested that other D-amino-acid-containing peptides might be present in the venom. In the present study, we show that DLP-2 (defensin-like peptide-2), a 42-amino-acid residue polypeptide in the platypus venom, also contains a D-amino acid residue, D-methionine, at position 2, while DLP-4, which has an identical amino acid sequence, has all amino acids in the L-form. These findings were supported further by the detection of isomerase activity in the platypus gland venom extract that converts DLP-4 into DLP-2. In the light of this new information, the tertiary structure of DLP-2 was recalculated using a new structural template with D-Met2. The structure of DLP-4 was also determined in order to evaluate the effect of a D-amino acid at position 2 on the structure and possibly to explain the large retention time difference observed for the two molecules in reverse-phase HPLC. The solution structures of the DLP-2 and DLP-4 are very similar to each other and to the earlier reported structure of DLP-2, which assumed that all amino acids were in the L-form. Our results suggest that the incorporation of the D-amino acid at position 2 has minimal effect on the overall fold in solution.
About this Structure
1ZUF is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
D-amino acid residue in a defensin-like peptide from platypus venom: effect on structure and chromatographic properties., Torres AM, Tsampazi C, Geraghty DP, Bansal PS, Alewood PF, Kuchel PW, Biochem J. 2005 Oct 15;391(Pt 2):215-20. PMID:16033333
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