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4ic1

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Revision as of 00:56, 5 August 2016

Crystal structure of SSO0001

Structural highlights

4ic1 is a 10 chain structure with sequence from Sulso. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
NonStd Res:
Gene:	SSO0001 (SULSO)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[CAS4_SULSO] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (By similarity). A 5' to 3' partially processive exonuclease. Has a marked preference for ssDNA, although in vitro it also acts on dsDNA and ssRNA.

Publication Abstract from PubMed

Cas4 proteins, a core protein family associated with the microbial system of adaptive immunity CRISPR, are predicted to function in the adaptation step of the CRISPR mechanism. Here we show that the Cas4 protein SSO0001 from the archaeon Sulfolobus solfataricus has metal-dependent endonuclease and 5'-->3' exonuclease activities against single-stranded DNA, as well as ATP-independent DNA unwinding activity toward double-stranded DNA. The crystal structure of SSO0001 revealed a decameric toroid formed by five dimers with each protomer containing one [4Fe-4S] cluster and one Mn(2+) ion bound in the active site located inside the internal tunnel. The conserved RecB motif and four Cys residues are important for DNA binding and cleavage activities, whereas DNA unwinding depends on several residues located near the [4Fe-4S] cluster. Our results suggest that Cas4 proteins might contribute to the addition of novel CRISPR spacers through the formation of 3'-DNA overhangs and to the degradation of foreign DNA.

Toroidal structure and DNA cleavage by the CRISPR-associated [4Fe-4S] cluster containing Cas4 nuclease SSO0001 from Sulfolobus solfataricus.,Lemak S, Beloglazova N, Nocek B, Skarina T, Flick R, Brown G, Popovic A, Joachimiak A, Savchenko A, Yakunin AF J Am Chem Soc. 2013 Nov 20;135(46):17476-87. doi: 10.1021/ja408729b. Epub 2013, Nov 11. PMID:24171432^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lemak S, Beloglazova N, Nocek B, Skarina T, Flick R, Brown G, Popovic A, Joachimiak A, Savchenko A, Yakunin AF. Toroidal structure and DNA cleavage by the CRISPR-associated [4Fe-4S] cluster containing Cas4 nuclease SSO0001 from Sulfolobus solfataricus. J Am Chem Soc. 2013 Nov 20;135(46):17476-87. doi: 10.1021/ja408729b. Epub 2013, Nov 11. PMID:24171432 doi:http://dx.doi.org/10.1021/ja408729b

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4ic1"

@@ Line 1: / Line 1: @@
 ==Crystal structure of SSO0001==
 <StructureSection load='4ic1' size='340' side='right' caption='[[4ic1]], [[Resolution|resolution]] 2.35&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SSO0001 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 SULSO])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ic1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ic1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ic1 RCSB], [http://www.ebi.ac.uk/pdbsum/4ic1 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ic1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ic1 OCA], [http://pdbe.org/4ic1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ic1 RCSB], [http://www.ebi.ac.uk/pdbsum/4ic1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ic1 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4ic1" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4ic1

From Proteopedia

Revision as of 00:56, 5 August 2016

Crystal structure of SSO0001

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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