1zx4
From Proteopedia
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|PDB= 1zx4 |SIZE=350|CAPTION= <scene name='initialview01'>1zx4</scene>, resolution 2.98Å | |PDB= 1zx4 |SIZE=350|CAPTION= <scene name='initialview01'>1zx4</scene>, resolution 2.98Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CIT:CITRIC ACID'>CIT</scene> | + | |LIGAND= <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene> |
|ACTIVITY= | |ACTIVITY= | ||
| - | |GENE= parb ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id= | + | |GENE= parb ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10678 Enterobacteria phage P1]) |
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zx4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zx4 OCA], [http://www.ebi.ac.uk/pdbsum/1zx4 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zx4 RCSB]</span> | ||
}} | }} | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1ZX4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ | + | 1ZX4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p1 Enterobacteria phage p1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZX4 OCA]. |
==Reference== | ==Reference== | ||
Structures of ParB bound to DNA reveal mechanism of partition complex formation., Schumacher MA, Funnell BE, Nature. 2005 Nov 24;438(7067):516-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16306995 16306995] | Structures of ParB bound to DNA reveal mechanism of partition complex formation., Schumacher MA, Funnell BE, Nature. 2005 Nov 24;438(7067):516-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16306995 16306995] | ||
| - | [[Category: Enterobacteria phage | + | [[Category: Enterobacteria phage p1]] |
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Funnell, B E.]] | [[Category: Funnell, B E.]] | ||
[[Category: Schumacher, M A.]] | [[Category: Schumacher, M A.]] | ||
| - | [[Category: | + | [[Category: p1]] |
| - | [[Category: partition | + | [[Category: partition]] |
| + | [[Category: plasmid]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:42:54 2008'' |
Revision as of 22:42, 30 March 2008
| |||||||
| , resolution 2.98Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , , | ||||||
| Gene: | parb (Enterobacteria phage P1) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of ParB bound to DNA
Overview
The faithful inheritance of genetic information, which is essential for all organisms, requires accurate DNA partition (segregation) at cell division. In prokaryotes, partition is mediated by par systems, for which the P1 plasmid system of Escherichia coli is a prototype comprising a partition site and two proteins, ParA and ParB. To form the partition complex necessary for segregation, P1 ParB must recognize a complicated arrangement of A-box and B-box DNA motifs located on opposite ends of a sharply bent parS partition site of approximately 74 bp (refs 3-7). Here we describe structures of ParB bound to partition sites. ParB forms an asymmetric dimer with extended amino-terminal HTH (helix-turn-helix) domains that contact A-boxes. The two HTH domains emanate from a dimerized DNA-binding module composed of a six-stranded beta-sheet coiled-coil that binds B-boxes. Strikingly, these individual DNA-binding modules rotate freely about a flexible linker, enabling them to contact several arrangements of A- and B-boxes. Most notably, each DNA-binding element binds to and thus bridges adjacent DNA duplexes. These unique structural features of ParB explain how this protein can bind complex arrays of A- and B-box elements on adjacent DNA arms of the looped partition site.
About this Structure
1ZX4 is a Single protein structure of sequence from Enterobacteria phage p1. Full crystallographic information is available from OCA.
Reference
Structures of ParB bound to DNA reveal mechanism of partition complex formation., Schumacher MA, Funnell BE, Nature. 2005 Nov 24;438(7067):516-9. PMID:16306995
Page seeded by OCA on Mon Mar 31 01:42:54 2008
