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4lrz

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Revision as of 01:47, 5 August 2016

Crystal Structure of the E.coli DhaR(N)-DhaL complex

Structural highlights

4lrz is a 8 chain structure with sequence from Ecoli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Related:	4lrx, 4lry
Gene:	b1199, dhaL, dhaR, JW5186, ycgS (ECOLI), b1201, dhaL, dhaR, JW5188, ycgU (ECOLI)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[DHAL_ECOLI] ADP-binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone. DhaL-ADP receives a phosphoryl group from DhaM and transmits it to dihydroxyacetone. DhaL-ADP acts also as a coactivator by binding to the sensor domain of DhaR. DhaL-ATP is inactive. [DHAR_ECOLI] Positively regulates the dhaKLM operon from a sigma-70 promoter. Represses its own synthesis.

Publication Abstract from PubMed

Escherichia coli dihydroxyacetone (Dha) kinase consists of two subunits, DhaK and DhaL. Transcription of dha operon is regulated by the DhaR transcription factor and its action is under control of the kinase subunits. DhaR is activated by interaction with DhaL while it is repressed by DhaK. We have determined the structures of DhaK and DhaL bound to the tandem GAF-like and PAS domains of the DhaR, providing an architectural model for how GAF/PAS tandem domains work together in binding protein partners. The structures reveal a mechanism of opposite transcriptional regulation by the DhaK and DhaL subunits. The kinase subunits interface with DhaR through surfaces that partially overlap with their active sites, allowing sensing of ATP- versus ADP-loaded DhaL subunit and also precluding a ternary complex between DhaK-DhaL and DhaR. The rotation of helices within the DhaR coiled-coil linker upon DhaL binding provides the mechanism for transmitting the binding signal from the GAF/PAS domains to the C-terminal DNA-binding domain.

Coiled-Coil Helix Rotation Selects Repressing or Activating State of Transcriptional Regulator DhaR.,Shi R, McDonald L, Cygler M, Ekiel I Structure. 2014 Jan 14. pii: S0969-2126(13)00471-1. doi:, 10.1016/j.str.2013.11.012. PMID:24440518^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Shi R, McDonald L, Cygler M, Ekiel I. Coiled-Coil Helix Rotation Selects Repressing or Activating State of Transcriptional Regulator DhaR. Structure. 2014 Jan 14. pii: S0969-2126(13)00471-1. doi:, 10.1016/j.str.2013.11.012. PMID:24440518 doi:http://dx.doi.org/10.1016/j.str.2013.11.012

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4lrz"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of the E.coli DhaR(N)-DhaL complex==
 <StructureSection load='4lrz' size='340' side='right' caption='[[4lrz]], [[Resolution|resolution]] 2.32&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4lrx|4lrx]], [[4lry|4lry]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">b1199, dhaL, dhaR, JW5186, ycgS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI]), b1201, dhaL, dhaR, JW5188, ycgU ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lrz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lrz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4lrz RCSB], [http://www.ebi.ac.uk/pdbsum/4lrz PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4lrz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4lrz OCA], [http://pdbe.org/4lrz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4lrz RCSB], [http://www.ebi.ac.uk/pdbsum/4lrz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4lrz ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4lrz" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4lrz

From Proteopedia

Revision as of 01:47, 5 August 2016

Crystal Structure of the E.coli DhaR(N)-DhaL complex

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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