1zyr
From Proteopedia
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|PDB= 1zyr |SIZE=350|CAPTION= <scene name='initialview01'>1zyr</scene>, resolution 3.00Å | |PDB= 1zyr |SIZE=350|CAPTION= <scene name='initialview01'>1zyr</scene>, resolution 3.00Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=STD:STREPTOLYDIGIN'>STD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1iw7|1IW7]], [[1smy|1SMY]], [[1ynj|1YNJ]], [[1ynn|1YNN]], [[1k83|1K83]], [[1i6h|1I6H]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zyr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zyr OCA], [http://www.ebi.ac.uk/pdbsum/1zyr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1zyr RCSB]</span> | ||
}} | }} | ||
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[[Category: Tuske, S.]] | [[Category: Tuske, S.]] | ||
[[Category: Wang, X.]] | [[Category: Wang, X.]] | ||
| - | [[Category: | + | [[Category: holoenzyme]] |
| - | [[Category: | + | [[Category: rna polymerase]] |
| - | [[Category: | + | [[Category: streptolydigin]] |
| - | [[Category: | + | [[Category: transcription]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:43:25 2008'' |
Revision as of 22:43, 30 March 2008
| |||||||
| , resolution 3.00Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Activity: | DNA-directed RNA polymerase, with EC number 2.7.7.6 | ||||||
| Related: | 1IW7, 1SMY, 1YNJ, 1YNN, 1K83, 1I6H
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of Thermus thermophilus RNA polymerase holoenzyme in complex with the antibiotic streptolydigin
Overview
We define the target, mechanism, and structural basis of inhibition of bacterial RNA polymerase (RNAP) by the tetramic acid antibiotic streptolydigin (Stl). Stl binds to a site adjacent to but not overlapping the RNAP active center and stabilizes an RNAP-active-center conformational state with a straight-bridge helix. The results provide direct support for the proposals that alternative straight-bridge-helix and bent-bridge-helix RNAP-active-center conformations exist and that cycling between straight-bridge-helix and bent-bridge-helix RNAP-active-center conformations is required for RNAP function. The results set bounds on models for RNAP function and suggest strategies for design of novel antibacterial agents.
About this Structure
1ZYR is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.
Reference
Inhibition of bacterial RNA polymerase by streptolydigin: stabilization of a straight-bridge-helix active-center conformation., Tuske S, Sarafianos SG, Wang X, Hudson B, Sineva E, Mukhopadhyay J, Birktoft JJ, Leroy O, Ismail S, Clark AD Jr, Dharia C, Napoli A, Laptenko O, Lee J, Borukhov S, Ebright RH, Arnold E, Cell. 2005 Aug 26;122(4):541-52. PMID:16122422
Page seeded by OCA on Mon Mar 31 01:43:25 2008
Categories: DNA-directed RNA polymerase | Protein complex | Thermus thermophilus | Arnold, E. | Birktoft, J J. | Borukhov, S. | Clark, A D. | Dharia, C. | Ebright, R H. | Hudson, B. | Ismail, S. | Laptenko, O. | Lee, J. | Leroy, O. | Mukhopadhyay, J. | Napoli, A. | Sarafianos, S G. | Sineva, E. | Tuske, S. | Wang, X. | Holoenzyme | Rna polymerase | Streptolydigin | Transcription
