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4axr

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Revision as of 02:02, 5 August 2016

CRYSTAL STRUCTURE OF thaumatin FROM A AUTO-HARVESTED CRYSTAL

Structural highlights

4axr is a 1 chain structure with sequence from Thaumatococcus daniellii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	1kwn, 1lr2, 1lr3, 1lxz, 1ly0, 1pp3, 1rqw, 1thi, 1thu, 1thv, 1thw, 2a7i, 2blr, 2blu, 2d8o, 2d8p, 2vhk, 2vhr, 2vi1, 2vi2, 2vi3, 2vi4, 2vu6, 2vu7, 2wbz, 4axu
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[THM1_THADA] Taste-modifying protein; intensely sweet-tasting. It is 100000 times sweeter than sucrose on a molar basis.

Publication Abstract from PubMed

The use of automated systems for crystallization and X-ray data collection is now widespread. However, these two steps are separated by the need to transfer crystals from crystallization supports to X-ray data-collection supports, which is a difficult manual operation. Here, a new approach is proposed called CrystalDirect (CD) which enables full automation of the crystal-harvesting process. In this approach, crystals are grown on ultrathin films in a newly designed vapour-diffusion crystallization plate and are recovered by excision of the film through laser-induced photoablation. The film pieces containing crystals are then directly attached to a pin for X-ray data collection. This new method eliminates the delicate step of `crystal fishing', thereby enabling full automation of the crystal-mounting process. Additional advantages of this approach include the absence of mechanical stress and that it facilitates handling of microcrystals. The CD crystallization plates are also suitable for in situ crystal screening with minimal X-ray background. This method could enable the operational integration of highly automated crystallization and data-collection facilities, minimizing the delay between crystal identification and diffraction measurements. It can also contribute significantly to the advancement of challenging projects that require the systematic testing of large numbers of crystals.

CrystalDirect: a new method for automated crystal harvesting based on laser-induced photoablation of thin films.,Cipriani F, Rower M, Landret C, Zander U, Felisaz F, Marquez JA Acta Crystallogr D Biol Crystallogr. 2012 Oct;68(Pt 10):1393-9. Epub 2012 Sep 18. PMID:22993093^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Cipriani F, Rower M, Landret C, Zander U, Felisaz F, Marquez JA. CrystalDirect: a new method for automated crystal harvesting based on laser-induced photoablation of thin films. Acta Crystallogr D Biol Crystallogr. 2012 Oct;68(Pt 10):1393-9. Epub 2012 Sep 18. PMID:22993093 doi:http://dx.doi.org/10.1107/S0907444912031459

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4axr"

@@ Line 1: / Line 1: @@
 ==CRYSTAL STRUCTURE OF thaumatin FROM A AUTO-HARVESTED CRYSTAL==
 <StructureSection load='4axr' size='340' side='right' caption='[[4axr]], [[Resolution|resolution]] 1.38&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1kwn|1kwn]], [[1lr2|1lr2]], [[1lr3|1lr3]], [[1lxz|1lxz]], [[1ly0|1ly0]], [[1pp3|1pp3]], [[1rqw|1rqw]], [[1thi|1thi]], [[1thu|1thu]], [[1thv|1thv]], [[1thw|1thw]], [[2a7i|2a7i]], [[2blr|2blr]], [[2blu|2blu]], [[2d8o|2d8o]], [[2d8p|2d8p]], [[2vhk|2vhk]], [[2vhr|2vhr]], [[2vi1|2vi1]], [[2vi2|2vi2]], [[2vi3|2vi3]], [[2vi4|2vi4]], [[2vu6|2vu6]], [[2vu7|2vu7]], [[2wbz|2wbz]], [[4axu|4axu]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4axr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4axr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4axr RCSB], [http://www.ebi.ac.uk/pdbsum/4axr PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4axr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4axr OCA], [http://pdbe.org/4axr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4axr RCSB], [http://www.ebi.ac.uk/pdbsum/4axr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4axr ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4axr" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4axr

From Proteopedia

Revision as of 02:02, 5 August 2016

CRYSTAL STRUCTURE OF thaumatin FROM A AUTO-HARVESTED CRYSTAL

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

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