208d
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 208d |SIZE=350|CAPTION= <scene name='initialview01'>208d</scene>, resolution 2.050Å | |PDB= 208d |SIZE=350|CAPTION= <scene name='initialview01'>208d</scene>, resolution 2.050Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene> | + | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=208d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=208d OCA], [http://www.ebi.ac.uk/pdbsum/208d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=208d RCSB]</span> | ||
}} | }} | ||
| Line 27: | Line 30: | ||
[[Category: Precigoux, G.]] | [[Category: Precigoux, G.]] | ||
[[Category: Vlieghe, D.]] | [[Category: Vlieghe, D.]] | ||
| - | [[Category: MG]] | ||
[[Category: b-dna]] | [[Category: b-dna]] | ||
[[Category: base triplet]] | [[Category: base triplet]] | ||
[[Category: double helix]] | [[Category: double helix]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:44:06 2008'' |
Revision as of 22:44, 30 March 2008
| |||||||
| , resolution 2.050Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
HIGH-RESOLUTION STRUCTURE OF A DNA HELIX FORMING (C.G)*G BASE TRIPLETS
Overview
Triple helices result from interaction between single- and double-stranded nucleic acids. Their formation is a possible mechanism for recombination of homologous gene sequences in nature and provides, inter alia, a basis for artificial control of gene activity. Triple-helix motifs have been extensively studied by a variety of techniques, but few high-resolution structural data are available. The only triplet structures characterized so far by X-ray diffraction were in protein-DNA complexes studied at about 3 A resolution. We report here the X-ray analysis of a DNA nonamer, d(GCGAATTCG), to a resolution of 2.05 A, in which the extended crystal structure contains (C.G)*G triplets as a fragment of triple helix. The guanosine-containing chains are in a parallel orientation. This arrangement is a necessary feature of models for homologous recombination which results ultimately in replacement of one length of DNA by another of similar sequence. The present-structure agrees with many published predictions of triplex organization, and provides an accurate representation of an element that allows sequence-specific association between single- and double-stranded nucleic acids.
About this Structure
208D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
High-resolution structure of a DNA helix forming (C.G)*G base triplets., Van Meervelt L, Vlieghe D, Dautant A, Gallois B, Precigoux G, Kennard O, Nature. 1995 Apr 20;374(6524):742-4. PMID:7715732
Page seeded by OCA on Mon Mar 31 01:44:06 2008
