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4f5s

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Revision as of 03:06, 5 August 2016

Crystal Structure of Bovine Serum Albumin

Structural highlights

4f5s is a 2 chain structure with sequence from Bos taurus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ALBU_BOVIN] Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.

Publication Abstract from PubMed

Serum albumin first appeared in early vertebrates and is present in the plasma of all mammals. Its canonical structure supported by a conserved set of disulfide bridges is maintained in all mammalian serum albumins and any changes in sequence are highly correlated with evolution of the species. Previous structural investigations of mammalian serum albumins have only concentrated on human serum albumin (HSA), most likely as a consequence of crystallization and diffraction difficulties. Here, the crystal structures of serum albumins isolated from bovine, equine and leporine blood plasma are reported. The structure of bovine serum albumin (BSA) was determined at 2.47 A resolution, two crystal structures of equine serum albumin (ESA) were determined at resolutions of 2.32 and 2.04 A, and that of leporine serum albumin (LSA) was determined at 2.27 A resolution. These structures were compared in detail with the structure of HSA. The ligand-binding pockets in BSA, ESA and LSA revealed different amino-acid compositions and conformations in comparison to HSA in some cases; however, much more significant differences were observed on the surface of the molecules. BSA, which is one of the most extensively utilized proteins in laboratory practice and is used as an HSA substitute in many experiments, exhibits only 75.8% identity compared with HSA. The higher resolution crystal structure of ESA highlights the binding properties of this protein because it includes several bound compounds from the crystallization solution that provide additional structural information about potential ligand-binding pockets.

Structures of bovine, equine and leporine serum albumin.,Bujacz A Acta Crystallogr D Biol Crystallogr. 2012 Oct;68(Pt 10):1278-89. Epub 2012 Sep, 13. PMID:22993082^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bujacz A. Structures of bovine, equine and leporine serum albumin. Acta Crystallogr D Biol Crystallogr. 2012 Oct;68(Pt 10):1278-89. Epub 2012 Sep, 13. PMID:22993082 doi:http://dx.doi.org/10.1107/S0907444912027047

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4f5s"

Categories: Bos taurus | Bujacz, A | Bujacz, G | Bovine serum albumin | Transport protein

@@ Line 1: / Line 1: @@
 ==Crystal Structure of Bovine Serum Albumin==
 <StructureSection load='4f5s' size='340' side='right' caption='[[4f5s]], [[Resolution|resolution]] 2.47&Aring;' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[4f5s]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F5S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F5S FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f5s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f5s RCSB], [http://www.ebi.ac.uk/pdbsum/4f5s PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f5s OCA], [http://pdbe.org/4f5s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4f5s RCSB], [http://www.ebi.ac.uk/pdbsum/4f5s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4f5s ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 16: / Line 17: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4f5s" style="background-color:#fffaf0;"></div>
 ==See Also==

4f5s

From Proteopedia

Revision as of 03:06, 5 August 2016

Crystal Structure of Bovine Serum Albumin

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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