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283d
From Proteopedia
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|PDB= 283d |SIZE=350|CAPTION= <scene name='initialview01'>283d</scene>, resolution 2.300Å | |PDB= 283d |SIZE=350|CAPTION= <scene name='initialview01'>283d</scene>, resolution 2.300Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=MN:MANGANESE (II) ION'>MN</scene> | + | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=283d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=283d OCA], [http://www.ebi.ac.uk/pdbsum/283d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=283d RCSB]</span> | ||
}} | }} | ||
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[[Category: Holbrook, S R.]] | [[Category: Holbrook, S R.]] | ||
[[Category: Pardi, A.]] | [[Category: Pardi, A.]] | ||
| - | [[Category: MN]] | ||
[[Category: curved]] | [[Category: curved]] | ||
[[Category: double helix]] | [[Category: double helix]] | ||
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[[Category: unusual rna]] | [[Category: unusual rna]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 01:45:52 2008'' |
Revision as of 22:45, 30 March 2008
| |||||||
| , resolution 2.300Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
A CURVED RNA HELIX INCORPORATING AN INTERNAL LOOP WITH G-A AND A-A NON-WATSON-CRICK BASE PAIRING
Overview
The crystal structure of the RNA dodecamer 5'-GGCC(GAAA)GGCC-3' has been determined from x-ray diffraction data to 2.3-A resolution. In the crystal, these oligomers form double helices around twofold symmetry axes. Four consecutive non-Watson-Crick base pairs make up an internal loop in the middle of the duplex, including sheared G.A pairs and novel asymmetric A.A pairs. This internal loop sequence produces a significant curvature and narrowing of the double helix. The helix is curved by 34 degrees from end to end and the diameter is narrowed by 24% in the internal loop. A Mn2+ ion is bound directly to the N7 of the first guanine in the Watson-Crick region following the internal loop and the phosphate of the preceding residue. This Mn2+ location corresponds to a metal binding site observed in the hammerhead catalytic RNA.
About this Structure
283D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
A curved RNA helix incorporating an internal loop with G.A and A.A non-Watson-Crick base pairing., Baeyens KJ, De Bondt HL, Pardi A, Holbrook SR, Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):12851-5. PMID:8917508 [[Category: ]]
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