2a3r
From Proteopedia
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|PDB= 2a3r |SIZE=350|CAPTION= <scene name='initialview01'>2a3r</scene>, resolution 2.60Å | |PDB= 2a3r |SIZE=350|CAPTION= <scene name='initialview01'>2a3r</scene>, resolution 2.60Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=A3P:ADENOSINE-3 | + | |LIGAND= <scene name='pdbligand=A3P:ADENOSINE-3'-5'-DIPHOSPHATE'>A3P</scene> and <scene name='pdbligand=LDP:L-DOPAMINE'>LDP</scene> |
|ACTIVITY= [http://en.wikipedia.org/wiki/Aryl_sulfotransferase Aryl sulfotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.1 2.8.2.1] | |ACTIVITY= [http://en.wikipedia.org/wiki/Aryl_sulfotransferase Aryl sulfotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.2.1 2.8.2.1] | ||
|GENE= | |GENE= | ||
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[[Category: sult1a3]] | [[Category: sult1a3]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 14:34:27 2008'' |
Revision as of 12:34, 23 March 2008
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| , resolution 2.60Å | |||||||
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| Ligands: | and | ||||||
| Activity: | Aryl sulfotransferase, with EC number 2.8.2.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Human Sulfotransferase SULT1A3 in Complex with Dopamine and 3-Phosphoadenosine 5-Phosphate
Contents |
Overview
The human sulfotransferase, SULT1A3, catalyzes specifically the sulfonation of monoamines such as dopamine, epinephrine, and norepinephrine. SULT1A3 also has a unique 3,4-dihydroxyphenylalanine (Dopa)/tyrosine-sulfating activity that is preferentially toward their D-form enantiomers and can be stimulated dramatically by Mn2+. To further our understanding of the molecular basis for the unique substrate specificity of this enzyme, we solved the crystal structure of human SULT1A3, complexed with dopamine and 3'-phosphoadenosine 5'-phosphate, at 2.6 A resolution and carried out autodocking analysis with D-Dopa. The structure of SULT1A3 enzyme-ligand complex clearly showed that residue Glu146 can form electrostatic interaction with dopamine and may play a pivotal role in the stereoselectivity and sulfating activity. On the other hand, residue Asp86 appeared to be critical to the Mn2+-stimulation of the Dopa/tyrosine-sulfating activity of SULT1A3, in addition to a supporting role in the stereoselectivity and sulfating activity.
Disease
Known diseases associated with this structure: Alzheimer disease-4 OMIM:[600759], Cardiomyopathy, dilated, 1V OMIM:[600759]
About this Structure
2A3R is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of human sulfotransferase SULT1A3 in complex with dopamine and 3'-phosphoadenosine 5'-phosphate., Lu JH, Li HT, Liu MC, Zhang JP, Li M, An XM, Chang WR, Biochem Biophys Res Commun. 2005 Sep 23;335(2):417-23. PMID:16083857
Page seeded by OCA on Sun Mar 23 14:34:27 2008
Categories: Aryl sulfotransferase | Homo sapiens | Single protein | An, X M. | Chang, W R. | Li, H T. | Li, M. | Liu, M C. | Lu, J H. | Zhang, J P. | A3P | LDP | Complex | Crystal structure | Dopamine | Sulfotransferase | Sult1a3
