1h6v

From Proteopedia

Revision as of 14:25, 5 November 2007

MAMMALIAN THIOREDOXIN REDUCTASE

Overview

Thioredoxin reductases (TrxRs) from mammalian cells contain an essential, selenocysteine residue in the conserved C-terminal sequence, Gly-Cys-SeCys-Gly forming a selenenylsulfide in the oxidized enzyme., Reduction by NADPH generates a selenolthiol, which is the active site in, reduction of Trx. The three-dimensional structure of the SeCys498Cys, mutant of rat TrxR in complex with NADP(+) has been determined to 3.0-A, resolution by x-ray crystallography. The overall structure is similar to, that of glutathione reductase (GR), including conserved amino acid, residues binding the cofactors FAD and NADPH. Surprisingly, all residues, directly interacting with the substrate glutathione disulfide in GR are, conserved despite the failure of glutathione disulfide to act as a, substrate for TrxR. The 16-residue C-terminal tail, which is unique to, mammalian TrxR, folds in such a way that it can approach the active site, disulfide of the other subunit in the dimer. A model of the complex of, TrxR with Trx suggests that electron transfer from NADPH to the disulfide, of the substrate is possible without large conformational changes. The, C-terminal extension typical of mammalian TrxRs has two functions: (i) it, extends the electron transport chain from the catalytic disulfide to the, enzyme surface, where it can react with Trx, and (ii) it prevents the, enzyme from acting as a GR by blocking the redox-active disulfide. Our, results suggest that mammalian TrxR evolved from the GR scaffold rather, than from its prokaryotic counterpart. This evolutionary switch renders, cell growth dependent on selenium.

About this Structure

1H6V is a Single protein structure of sequence from Rattus norvegicus with FAD and NDP as ligands. Active as Transferred entry: 1.8.1.9, with EC number 1.6.4.5 Structure known Active Sites: FAA, FAB, FAC, FAD, FAE, FAF, NDA, NDB, NDC, NDD, NDE and NDF. Full crystallographic information is available from OCA.

Reference

Three-dimensional structure of a mammalian thioredoxin reductase: implications for mechanism and evolution of a selenocysteine-dependent enzyme., Sandalova T, Zhong L, Lindqvist Y, Holmgren A, Schneider G, Proc Natl Acad Sci U S A. 2001 Aug 14;98(17):9533-8. Epub 2001 Jul 31. PMID:11481439

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