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3nj5

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Revision as of 06:43, 5 August 2016

Crystal structure of chicken IL-1 hydrophobic cavity mutant 157

Structural highlights

3nj5 is a 1 chain structure with sequence from Chick. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[O73909_CHICK] Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells (By similarity).[RuleBase:RU003753]

Publication Abstract from PubMed

Interleukin-1 beta (IL-1beta) is an important cytokine in the immune system. The properties of avian IL-1betas are less well understood than the mammalian IL-1betas, and there is no available structure of avian IL-1betas in the Protein Data Bank. Here, we report the crystal structures of wild-type and Y157F mutant IL-1betas from chicken. Both the wild-type and mutant IL-1betas share a beta-trefoil conformation similar to that of human IL-1beta and also have an internal hydrophobic cavity. However, the cavity sizes clearly differ from that of human IL-1beta due to the packing of hydrophobic residues. Our studies also reveal that the relative thermal stability of IL-1betas does not correlate with cavity size but rather is dependent on the amino acid residues present around the cavity. This cavity serves as a scaffold for maintaining the structure of the IL-1beta core region but does not have a biological function per se. Moreover, we found that human IL-1beta cannot induce chemokine expression in chicken fibroblasts or elevate plasma cortisol levels in chickens, implying a lack of cross-species bioactivity. Close examination reveals that significant structural and sequence differences occur in the terminal and some loop regions between human and chicken IL-1betas. These variable regions have been shown to be critical for receptor binding, thus resulting in a lack of species cross-reactivity between human and chicken IL-1beta.

Structural and functional comparison of cytokine interleukin-1 beta from chicken and human.,Cheng CS, Chen WT, Lee LH, Chen YW, Chang SY, Lyu PC, Yin HS Mol Immunol. 2011 Mar;48(6-7):947-55. Epub 2011 Feb 1. PMID:21288573^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Cheng CS, Chen WT, Lee LH, Chen YW, Chang SY, Lyu PC, Yin HS. Structural and functional comparison of cytokine interleukin-1 beta from chicken and human. Mol Immunol. 2011 Mar;48(6-7):947-55. Epub 2011 Feb 1. PMID:21288573 doi:10.1016/j.molimm.2011.01.002

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3nj5"

@@ Line 1: / Line 1: @@
 ==Crystal structure of chicken IL-1 hydrophobic cavity mutant 157==
 <StructureSection load='3nj5' size='340' side='right' caption='[[3nj5]], [[Resolution|resolution]] 1.67&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3nj5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NJ5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NJ5 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3nj5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chick Chick]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NJ5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NJ5 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nj5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nj5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3nj5 RCSB], [http://www.ebi.ac.uk/pdbsum/3nj5 PDBsum]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nj5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nj5 OCA], [http://pdbe.org/3nj5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3nj5 RCSB], [http://www.ebi.ac.uk/pdbsum/3nj5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3nj5 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 15: / Line 16: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3nj5" style="background-color:#fffaf0;"></div>
 ==See Also==
@@ Line 22: / Line 24: @@
 __TOC__
 </StructureSection>
-[[Category: Gallus gallus]]
+[[Category: Chick]]
 [[Category: Chen, Y W]]
 [[Category: Yin, H S]]

3nj5

From Proteopedia

Revision as of 06:43, 5 August 2016

Crystal structure of chicken IL-1 hydrophobic cavity mutant 157

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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