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4fcm

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Revision as of 07:52, 5 August 2016

Crystal structure of the NTF2-like domain of human G3BP1 in complex with a peptide

Structural highlights

4fcm is a 4 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	3ujm, 3q90, 4fcj
Gene:	G3BP1, G3BP (HUMAN)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[G3BP1_HUMAN] May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.^[1] ^[2]

Publication Abstract from PubMed

Ras GTPase Activating Protein SH3 Domain Binding Protein (G3BP) is a potential anti-cancer drug target implicated in several cellular functions. We have used protein crystallography to solve crystal structures of the human G3BP1 NTF2-like domain both alone and in complex with an FxFG Nup repeat peptide. Despite high structural similarity, the FxFG binding site is located between two alpha helices in the G3BP1 NTF2-like domain and not at the dimer interface as observed for nuclear transport factor 2. ITC studies showed specificity towards the FxFG motif but not FG and GLFG motifs. The unliganded form of the G3BP1 NTF2-like domain was solved in two crystal forms to resolutions of 1.6 and 3.3 A in space groups P212121 and P6322 based on two different constructs, residues 1-139 and 11-139, respectively. Crystal packing of the N-terminal residues against a symmetry related molecule in the P212121 crystal form might indicate a novel ligand binding site that, however, remains to be validated. The crystal structures give insight into the nuclear transportation mechanisms of G3BP and provide a basis for future structure based drug design.

Crystal Structures of the Human G3BP1 NTF2-Like Domain Visualize FxFG Nup Repeat Specificity.,Vognsen T, Moller IR, Kristensen O PLoS One. 2013 Dec 4;8(12):e80947. doi: 10.1371/journal.pone.0080947. PMID:24324649^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Costa M, Ochem A, Staub A, Falaschi A. Human DNA helicase VIII: a DNA and RNA helicase corresponding to the G3BP protein, an element of the ras transduction pathway. Nucleic Acids Res. 1999 Feb 1;27(3):817-21. PMID:9889278
↑ Tourriere H, Gallouzi IE, Chebli K, Capony JP, Mouaikel J, van der Geer P, Tazi J. RasGAP-associated endoribonuclease G3Bp: selective RNA degradation and phosphorylation-dependent localization. Mol Cell Biol. 2001 Nov;21(22):7747-60. PMID:11604510 doi:10.1128/MCB.21.22.7747-7760.2001
↑ Vognsen T, Moller IR, Kristensen O. Crystal Structures of the Human G3BP1 NTF2-Like Domain Visualize FxFG Nup Repeat Specificity. PLoS One. 2013 Dec 4;8(12):e80947. doi: 10.1371/journal.pone.0080947. PMID:24324649 doi:http://dx.doi.org/10.1371/journal.pone.0080947

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4fcm"

Categories: Human | Kristensen, O | Vognsen, T | Hydrolase | Ntf2-like domain

@@ Line 1: / Line 1: @@
 ==Crystal structure of the NTF2-like domain of human G3BP1 in complex with a peptide==
 <StructureSection load='4fcm' size='340' side='right' caption='[[4fcm]], [[Resolution|resolution]] 2.69&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3ujm|3ujm]], [[3q90|3q90]], [[4fcj|4fcj]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">G3BP1, G3BP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fcm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fcm OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fcm RCSB], [http://www.ebi.ac.uk/pdbsum/4fcm PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fcm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fcm OCA], [http://pdbe.org/4fcm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fcm RCSB], [http://www.ebi.ac.uk/pdbsum/4fcm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fcm ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4fcm" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

4fcm

From Proteopedia

Revision as of 07:52, 5 August 2016

Crystal structure of the NTF2-like domain of human G3BP1 in complex with a peptide

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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