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3vod

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Revision as of 08:13, 5 August 2016

Crystal Structure of mutant MarR C80S from E.coli

Structural highlights

3vod is a 2 chain structure with sequence from Ecoli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	3voe
Gene:	b1530, cfxB, inaR, JW5248, marR, soxQ (ECOLI)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MARR_ECOLI] Repressor of the marRAB operon which is involved in the activation of both antibiotic resistance and oxidative stress genes. Binds to the marO operator/promoter site.

Publication Abstract from PubMed

The widely conserved multiple antibiotic resistance regulator (MarR) family of transcription factors modulates bacterial detoxification in response to diverse antibiotics, toxic chemicals or both. The natural inducer for Escherichia coli MarR, the prototypical transcription repressor within this family, remains unknown. Here we show that copper signaling potentiates MarR derepression in E. coli. Copper(II) oxidizes a cysteine residue (Cys80) on MarR to generate disulfide bonds between two MarR dimers, thereby inducing tetramer formation and the dissociation of MarR from its cognate promoter DNA. We further discovered that salicylate, a putative MarR inducer, and the clinically important bactericidal antibiotics norfloxacin and ampicillin all stimulate intracellular copper elevation, most likely through oxidative impairment of copper-dependent envelope proteins, including NADH dehydrogenase-2. This membrane-associated copper oxidation and liberation process derepresses MarR, causing increased bacterial antibiotic resistance. Our study reveals that this bacterial transcription regulator senses copper(II) as a natural signal to cope with stress caused by antibiotics or the environment.

The multiple antibiotic resistance regulator MarR is a copper sensor in Escherichia coli.,Hao Z, Lou H, Zhu R, Zhu J, Zhang D, Zhao BS, Zeng S, Chen X, Chan J, He C, Chen PR Nat Chem Biol. 2014 Jan;10(1):21-8. doi: 10.1038/nchembio.1380. Epub 2013 Nov 3. PMID:24185215^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hao Z, Lou H, Zhu R, Zhu J, Zhang D, Zhao BS, Zeng S, Chen X, Chan J, He C, Chen PR. The multiple antibiotic resistance regulator MarR is a copper sensor in Escherichia coli. Nat Chem Biol. 2014 Jan;10(1):21-8. doi: 10.1038/nchembio.1380. Epub 2013 Nov 3. PMID:24185215 doi:http://dx.doi.org/10.1038/nchembio.1380

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3vod"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of mutant MarR C80S from E.coli==
 <StructureSection load='3vod' size='340' side='right' caption='[[3vod]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3voe|3voe]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">b1530, cfxB, inaR, JW5248, marR, soxQ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vod OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vod RCSB], [http://www.ebi.ac.uk/pdbsum/3vod PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vod OCA], [http://pdbe.org/3vod PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3vod RCSB], [http://www.ebi.ac.uk/pdbsum/3vod PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3vod ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 17: / Line 18: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 3vod" style="background-color:#fffaf0;"></div>
 == References ==
 <references/>

3vod

From Proteopedia

Revision as of 08:13, 5 August 2016

Crystal Structure of mutant MarR C80S from E.coli

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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