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4hfu

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Revision as of 08:47, 5 August 2016

Crystal structure of Fab 8M2 in complex with a H2N2 influenza virus hemagglutinin

Structural highlights

4hfu is a 4 chain structure with sequence from Human and Influenza a virus (a/japan/305+/1957(h2n2)). Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	4hg4
Gene:	HA (Influenza A virus (A/Japan/305+/1957(H2N2)))
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[Q67085_9INFA] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore (By similarity).[RuleBase:RU003324][SAAS:SAAS013829_004_327643]

Publication Abstract from PubMed

Influenza virus hemagglutinin (HA) mediates receptor binding and viral entry during influenza infection. The development of receptor analogs as viral-entry blockers has not been successful, which suggests that sialic acid may not be an ideal scaffold to obtain broad, potent HA inhibitors. Here, we report crystal structures of Fab fragments from three human antibodies that neutralize the 1957 pandemic H2N2 influenza virus in complex with H2 HA. All three antibodies use an aromatic residue to plug a conserved cavity in the HA receptor-binding site. Each antibody interacts with the absolutely conserved HA1 Trp153 at the cavity base through pi-pi stacking with the signature Phe54 of two VH1-69-encoded antibodies or a tyrosine from HCDR3 in the other antibody. This highly conserved interaction can be used as a starting point to design inhibitors targeting this conserved hydrophobic pocket in influenza viruses.

A recurring motif for antibody recognition of the receptor-binding site of influenza hemagglutinin.,Xu R, Krause JC, McBride R, Paulson JC, Crowe JE Jr, Wilson IA Nat Struct Mol Biol. 2013 Mar;20(3):363-70. doi: 10.1038/nsmb.2500. Epub 2013 Feb, 10. PMID:23396351^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Xu R, Krause JC, McBride R, Paulson JC, Crowe JE Jr, Wilson IA. A recurring motif for antibody recognition of the receptor-binding site of influenza hemagglutinin. Nat Struct Mol Biol. 2013 Mar;20(3):363-70. doi: 10.1038/nsmb.2500. Epub 2013 Feb, 10. PMID:23396351 doi:10.1038/nsmb.2500

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4hfu"

Categories: Human | Wilson, I A | Xu, R | Viral protein-immune system complex | Viral protein/immune system

@@ Line 1: / Line 1: @@
 ==Crystal structure of Fab 8M2 in complex with a H2N2 influenza virus hemagglutinin==
 <StructureSection load='4hfu' size='340' side='right' caption='[[4hfu]], [[Resolution|resolution]] 3.11&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4hfu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Influenza_a_virus_(a/japan/305+/1957(h2n2)) Influenza a virus (a/japan/305+/1957(h2n2))]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HFU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HFU FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4hfu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human] and [http://en.wikipedia.org/wiki/Influenza_a_virus_(a/japan/305+/1957(h2n2)) Influenza a virus (a/japan/305+/1957(h2n2))]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HFU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HFU FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4hg4|4hg4]]</td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=382813 Influenza A virus (A/Japan/305+/1957(H2N2))])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hfu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hfu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4hfu RCSB], [http://www.ebi.ac.uk/pdbsum/4hfu PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hfu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hfu OCA], [http://pdbe.org/4hfu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hfu RCSB], [http://www.ebi.ac.uk/pdbsum/4hfu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hfu ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 18: / Line 19: @@
 From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 </div>
+<div class="pdbe-citations 4hfu" style="background-color:#fffaf0;"></div>
 ==See Also==
@@ Line 25: / Line 27: @@
 __TOC__
 </StructureSection>
-[[Category: Homo sapiens]]
+[[Category: Human]]
 [[Category: Wilson, I A]]
 [[Category: Xu, R]]
 [[Category: Viral protein-immune system complex]]
 [[Category: Viral protein/immune system]]

4hfu

From Proteopedia

Revision as of 08:47, 5 August 2016

Crystal structure of Fab 8M2 in complex with a H2N2 influenza virus hemagglutinin

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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